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2A FINALS > BACTE LAB LA13 > Flashcards

BACTE LAB LA13 Flashcards

1
Q

a biochemical test used to differentiate Gram negative enteric bacteria based on carbohydrate fermentation, gas production, and hydrogen sulfide production

A

TRIPLE SUGAR IRON TEST

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2
Q

Carbohydrates included in the TSIA test

A

0.1% GLUCOSE
1% LACTOSE
1% SUCROSE

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3
Q

Identify the TSIA REACTION:

Alkaline slant over Acid Butt

A

Glucose Fermenters (K/A)

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4
Q

Identify the TSIA REACTION:

Acid Slant over Acid Butt

A

Glucose, Lactose and/ or Sucrose Fermenters (A/A)

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5
Q

Glucose, Lactose and/ or Sucrose Fermenters (A/A) Example bacteria

A

Enterobacter aerogenes,
Enterobacter cloacae,
Escherichia coli,
Klebsiella oxytoca,
Klebsiella pneumoniae.

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6
Q

Glucose Fermenters (K/A)
Example bacteria

A

Citrobacter freundii, Citrobacter koseri, Morganella morganii

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7
Q

Identify the TSIA REACTION:

Red Slant, Red Butt or Red Slant, No Change in Butt

A

NONFERMENTERS (K/K, K/NC)

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8
Q

Peptones are metabolized both aerobically and anaerobically

A

K/K Non Fermenter

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9
Q

Peptones are metabolized aerobically only

A

K/NC Non fermenter

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10
Q

No carbohydrates are fermented, instead, peptones are utilized, increasing pH

A

NONFERMENTERS (K/K, K/NC)

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11
Q

NONFERMENTERS (K/K, K/NC) example bacteria

A

Acinetobacter spp.
Pseudomonas spp.

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12
Q

Identify the TSIA REACTION:

Presence of gas bubbles, cracks, or agar displacement

A

Gas Production

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13
Q

Example for Bacteria that are capable of Gas Production

A

Enterobacter aerogenes,
Enterobacter cloacae,
Escherichia coli,
Klebsiella oxytoca,
Klebsiella pneumoniae.

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14
Q

Identify the TSIA REACTION:

Alkaline slant over Acid Butt with Black precipitate

A

Glucose Fermenter with H2S Production (K/A, H2S (+))

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15
Q

Substance in TSIA Test that allows for the detection of H2S Production

A

Sodium Thiosulfate

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16
Q

TSIA Test pH indicator

A

Phenol Red

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17
Q

Glucose Fermenter with H2S Production (K/A, H2S (+)) example bacteria

A

Citrobacter freundii, Edwardsiella tarda, Proteus mirabilis,
Salmonella spp.

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18
Q

Identify the TSIA REACTION:

Acid slant over Acid Butt with Black precipitate

A

Lactose, Sucrose, Glucose Fermenter with H2S Production (A/A, H2S (+))

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19
Q

Lactose, Sucrose, Glucose Fermenter with H2S Production (A/A, H2S (+)) example bacteria

A

Citrobacter freundii, Edwardsiella tarda, Proteus vulgaris

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20
Q

Identify the TSIA REACTION:

Alkaline slant over no change with Black precipitate

A

Nonfermenter with H2S production (K/NC, H2S (+))

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21
Q

Nonfermenter with H2S production (K/NC, H2S (+)) example bacteria

A

Shewanella spp.

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22
Q

a biochemical test used to differentiate bacteria based on their ability to decarboxylate, or deaminate lysine.

A

Lysine Iron Agar Test

23
Q

If the organism ferments glucose, the medium turbs yellow in the butt due to acid production

A

Glucose Fermentation

24
Q

Purple or Alkaline butt in LIA test

A

Lysine Decarboxylation

25
Burgundy color on the slant in LIA test
Lysine Deamination
26
H2S detection reagent in LIA
ferric ammonium citrate
27
Positive reaction for Lysine Decarboxylation
Purple Slant/ Purple butt
28
Positive reaction for Lysine Deamination
Red Slant
29
used as a part of the IMVIC tests to differentiate Enterobacteriaceae on their ability to use citrate as the sole carbon source
Simmons Citrate Agar Test
30
Positive Reaction for Citrate Test
Visible bacterial growth on slant, Medium Change from green to intense Prussian blue
31
a test that is a combination differential medium designed to assess three bacterial characteristics: sulfur reduction, indole production, and motility
SIM test
32
This test detects the ability of bacteria to reduce sulfur-containing compounds to hydrogen sulfide
Sulfur Reduction Test
33
Positive reaction for Sulfur Reduction Test
Blackening of the Media
34
Positive reaction for Indole Test
Cherry Red Ring in the Reagent Layer
35
This test differentiates bacteria based on their fermentation pathways
MRVP Test
36
detects mixed acid fermentation, which produces high levels of stable acids, significantly lowering the pH
Methyl Red Test
37
Positive reaction for MR Test
Red color change upon addition of Methyl red indicator, indicating a pH of <4.4
38
Common MR positive bacteria
E coli
39
Test that detects butanediol fermentation, where acetoin is converted into 2,3- butanediol
Voges-Proskauer test
40
Positive Reaction for VP Test
Pink to red coloration after adding a-naphthol and KOG due to the oxidation of acetoin to diacetyl
41
Common VP positive bacteria
Enterobacter aerogenes, Klebsiella pneumoniae
42
Test that detects the ability of bacteria to hydrolyze urea into ammonia and CO2 using a specific enzyme
UREASE TEST
43
Urease Test pH indicator
Phenol Red
44
Positive reaction for Urease Test
Bright pink color change due to alkaline pH
45
Common Urease positive bacteria
Proteus spp., Klebsiella spp.
46
Test that determines the bacterial ability to ferment specific carbohydrates with acid and gas production
Carbohydrate Fermentation Test
47
Positive reaction for Carbohydrate Fermentation Test
Medium color change indicating Acid Production
48
Test that determines the bacterial ability to decarboxylase, deaminate, or hydrolyze amino acids
Amino Acid Utilization Test
49
Positive reaction for Amino Acid Utilization Test
Purple Color
50
Common Lysine Decarboxylating organisms
Klebsiella pneumoniae Salmonella typhi Morganella morganii
51
Common Ornithine Decarboxylating organisms
Enterobacter cloacae Serratia marcescens Proteus mirabilis
52
Common Arginine Decarboxylating organisms
Enterococcus faecalis, Staphylococcus aureus
53
Identifies the bacteria capable of oxidative deamination of phenylalanine
Phenylalanine Deamination Test
54
common Phenylalanine deamination positive bacteria
Proteus spp., Morganella spp. Providencia spp.

2A FINALS (13 decks)

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