BACTE LAB LA13 Flashcards
a biochemical test used to differentiate Gram negative enteric bacteria based on carbohydrate fermentation, gas production, and hydrogen sulfide production
TRIPLE SUGAR IRON TEST
Carbohydrates included in the TSIA test
0.1% GLUCOSE
1% LACTOSE
1% SUCROSE
Identify the TSIA REACTION:
Alkaline slant over Acid Butt
Glucose Fermenters (K/A)
Identify the TSIA REACTION:
Acid Slant over Acid Butt
Glucose, Lactose and/ or Sucrose Fermenters (A/A)
Glucose, Lactose and/ or Sucrose Fermenters (A/A) Example bacteria
Enterobacter aerogenes,
Enterobacter cloacae,
Escherichia coli,
Klebsiella oxytoca,
Klebsiella pneumoniae.
Glucose Fermenters (K/A)
Example bacteria
Citrobacter freundii, Citrobacter koseri, Morganella morganii
Identify the TSIA REACTION:
Red Slant, Red Butt or Red Slant, No Change in Butt
NONFERMENTERS (K/K, K/NC)
Peptones are metabolized both aerobically and anaerobically
K/K Non Fermenter
Peptones are metabolized aerobically only
K/NC Non fermenter
No carbohydrates are fermented, instead, peptones are utilized, increasing pH
NONFERMENTERS (K/K, K/NC)
NONFERMENTERS (K/K, K/NC) example bacteria
Acinetobacter spp.
Pseudomonas spp.
Identify the TSIA REACTION:
Presence of gas bubbles, cracks, or agar displacement
Gas Production
Example for Bacteria that are capable of Gas Production
Enterobacter aerogenes,
Enterobacter cloacae,
Escherichia coli,
Klebsiella oxytoca,
Klebsiella pneumoniae.
Identify the TSIA REACTION:
Alkaline slant over Acid Butt with Black precipitate
Glucose Fermenter with H2S Production (K/A, H2S (+))
Substance in TSIA Test that allows for the detection of H2S Production
Sodium Thiosulfate
TSIA Test pH indicator
Phenol Red
Glucose Fermenter with H2S Production (K/A, H2S (+)) example bacteria
Citrobacter freundii, Edwardsiella tarda, Proteus mirabilis,
Salmonella spp.
Identify the TSIA REACTION:
Acid slant over Acid Butt with Black precipitate
Lactose, Sucrose, Glucose Fermenter with H2S Production (A/A, H2S (+))
Lactose, Sucrose, Glucose Fermenter with H2S Production (A/A, H2S (+)) example bacteria
Citrobacter freundii, Edwardsiella tarda, Proteus vulgaris
Identify the TSIA REACTION:
Alkaline slant over no change with Black precipitate
Nonfermenter with H2S production (K/NC, H2S (+))
Nonfermenter with H2S production (K/NC, H2S (+)) example bacteria
Shewanella spp.
a biochemical test used to differentiate bacteria based on their ability to decarboxylate, or deaminate lysine.
Lysine Iron Agar Test
If the organism ferments glucose, the medium turbs yellow in the butt due to acid production
Glucose Fermentation
Purple or Alkaline butt in LIA test
Lysine Decarboxylation
Burgundy color on the slant in LIA test
Lysine Deamination
H2S detection reagent in LIA
ferric ammonium citrate
Positive reaction for Lysine Decarboxylation
Purple Slant/ Purple butt
Positive reaction for Lysine Deamination
Red Slant
used as a part of the IMVIC tests to differentiate Enterobacteriaceae on their ability to use citrate as the sole carbon source
Simmons Citrate Agar Test
Positive Reaction for Citrate Test
Visible bacterial growth on slant, Medium Change from green to intense Prussian blue
a test that is a combination differential medium designed to assess three bacterial characteristics: sulfur reduction, indole production, and motility
SIM test
This test detects the ability of bacteria to reduce sulfur-containing compounds to hydrogen sulfide
Sulfur Reduction Test
Positive reaction for Sulfur Reduction Test
Blackening of the Media
Positive reaction for Indole Test
Cherry Red Ring in the Reagent Layer
This test differentiates bacteria based on their fermentation pathways
MRVP Test
detects mixed acid fermentation, which produces high levels of stable acids, significantly lowering the pH
Methyl Red Test
Positive reaction for MR Test
Red color change upon addition of Methyl red indicator, indicating a pH of <4.4
Common MR positive bacteria
E coli
Test that detects butanediol fermentation, where acetoin is converted into 2,3- butanediol
Voges-Proskauer test
Positive Reaction for VP Test
Pink to red coloration after adding a-naphthol and KOG due to the oxidation of acetoin to diacetyl
Common VP positive bacteria
Enterobacter aerogenes, Klebsiella pneumoniae
Test that detects the ability of bacteria to hydrolyze urea into ammonia and CO2 using a specific enzyme
UREASE TEST
Urease Test pH indicator
Phenol Red
Positive reaction for Urease Test
Bright pink color change due to alkaline pH
Common Urease positive bacteria
Proteus spp., Klebsiella spp.
Test that determines the bacterial ability to ferment specific carbohydrates with acid and gas production
Carbohydrate Fermentation Test
Positive reaction for Carbohydrate Fermentation Test
Medium color change indicating Acid Production
Test that determines the bacterial ability to decarboxylase, deaminate, or hydrolyze amino acids
Amino Acid Utilization Test
Positive reaction for Amino Acid Utilization Test
Purple Color
Common Lysine Decarboxylating organisms
Klebsiella pneumoniae
Salmonella typhi
Morganella morganii
Common Ornithine Decarboxylating organisms
Enterobacter cloacae
Serratia marcescens
Proteus mirabilis
Common Arginine Decarboxylating organisms
Enterococcus faecalis, Staphylococcus aureus
Identifies the bacteria capable of oxidative deamination of phenylalanine
Phenylalanine Deamination Test
common Phenylalanine deamination positive bacteria
Proteus spp., Morganella spp. Providencia spp.
