B5 - Enzymes

Question 1

Enzymes

Answer 1

Proteins that are involved in all metabolic reactions, where they function as biological catalysts

Question 2

What if the effect of temps too high or too low on enzymes

Answer 2

Low - enzyme activity is very low
High - enzymes denature meaning the shape of the active site changes

Question 3

Enzyme action

Answer 3

1 - substate molecule fits into active site
2 - enzyme-substrate complex is formed, the bond in the substrate weakens and breaks
3 - two smaller product molecules are made
4 - enzyme can then go and break down other substrates

Question 4

Why are enzymes’ specificity important

Answer 4

Each type of enzyme’s active site is complimentary to the shape of the substrate which it will break down

Question 5

What is the effect of changes in temp on enzyme activity

Answer 5

• high - enzyme denatures, changing shape of active site meaning enzyme cannot function
• optimum - the enzymes have a good supply of kinetic energy and therefore making the frequency of (effective) collision more, meaning the rate of enzyme activity increases
• low - the rate of enzyme activity is low as the enzyme has less kinetic energy and that means there are less frequent (effective) collisions

Question 6

What is the effect of changes in pH on enzyme activity

Answer 6

If the enzyme is not in its optimum pH the activity of the enzyme slows down and then stops

Question 7

What is the optimum pH of lipase

Answer 7

6 - 9

Question 8

What is the optimum pH of the 2 dif protease

Answer 8

Trypsin - 8
Pepsin - 2

Question 9

What is the optimum pH of amylase

Answer 9

7