B2 S1P

Question 1

S1P
what kind of molecule is S1P
what does it regulate
what does it promote

Answer 1

bioactive lipid
Regulates cell proliferation, differentiation, survival, and motility.
cancer growth

Question 2

what are the 2 mode of action of S1P

describe intracellular & extracellular actions

Answer 2

1. Intracellular 2nd messenger: Ca2+ mobilization.
2. Extracellular (agonist): via EDG (endothelial differentiation gene) receptors

S1P can function inside the cells as 2nd messenger OR is released from cells to act on 5 different S1P receptors

Question 3

S1PR KO has

Answer 3

1. disrupted cardiovascular development

Question 4

Sphingosine 1-phosphate is involved in what (3)

Answer 4

1. Vascular and airway biology
2. immune reactivity: S1P promotes T-lymphocyte egress
3. stem cell cancer research

Question 5

what is FTY720

Answer 5

fingolimod = S1PR functional antagonist
used to treat MS-FDA-Gilenya
- FTY720 binds to S1P1 on T-cells to promote internalisation of the receptor and degradation by the proteasome. (reduces Tc)

binds to S1P receptors, in the case of S1P1:

• S1P1 is removed from cell surface/ T cell cant leave lymph –> prevent auto reactive T cells from entering CNS
• -> immunosuppression

shown to reduce symptoms/ prevent progression in MS

Question 6

S1P does what to its receptor once activated

Answer 6

S1P activates S1P1 receptor then internalises it by: endocytosis of the recepto

Question 7

S1P activates ERK by which receptor

what does erk cause breast cancer cells to do

Answer 7

1. S1P3 receptor

2. migrate

Question 8

what Phosphorylates FTY720

Answer 8

SK2 to become FTY720-P

Question 9

is it possible to reverse effects of MS

Answer 9

can prevent in some cases if caught earlier enough and T cells remyelinated (can only be done if not already dead)