B cell lecture 1

Question 1

B cell activity

Answer 1

Humoral Activity

Question 2

Derived from

Answer 2

Bone Marrow

Question 3

Recognition of antigen

Answer 3

B cell receptor (BCR)

Question 4

Memory

Answer 4

Long term

Question 5

Effector molecules

Answer 5

Antibodies

Question 6

B cell structure

Answer 6

Large nucleus
Antibodies

Question 7

Naive B cell

Answer 7

Particular BCR
Particular antigen specificity

Question 8

How naive B cell activated?

Answer 8

Complement antigen protein

Question 9

Activated B cell differentiates in to

Answer 9

Memory Cell
Plasma (effector cells)

Question 10

Memory Cell

Answer 10

Longer life span
Same BCR of parent cells
Bone Marrow
Immunological Memory

Question 11

Plasma cell

Answer 11

Live for few days
Secrete antibodies (detachment)

Question 12

BCR

Answer 12

Has membrane bound antibody

Question 13

Structure of BCR

Answer 13

Variable Region
Constant Region
2 antigen binding sites

Question 14

Light chain

Answer 14

25Kda

Question 15

Heavy chain

Answer 15

50Kda

Question 16

Variable Region

Answer 16

2 X fab

Question 17

Constant Region

Answer 17

FC

Question 18

Isotypes of BCR

Answer 18

FC
Antobody determined by the carboxy terminus of the heavy chain

Question 19

Hinge Region of BCR

Answer 19

Disulfide Bond

Question 20

Antigen binding sites

Answer 20

CDRs
6 CDRs each

Question 21

CDR

Answer 21

Complementary Determining Regions

Question 22

CDR1 and CDR2

Answer 22

V gene segments

Question 23

CDR3

Answer 23

3 gene segments (V,D J)
Most variable loop
Heavy chain

Question 24

Diversity

Answer 24

VDJ recombination

Question 25

VDJ recombination

Answer 25

1. Germ line DNA: Gene segments
   L V J : light chain
   D : heavy chain
   -> D J come together

Question 26

Most diversity

Answer 26

V J

Question 27

Recombination V J

Answer 27

DNA level

Question 28

How does recombination occur?

Answer 28

Recombination Signal Sequences (RSSs)

Question 29

RSSs

Answer 29

Heptamer

Question 30

VDJ diversity

Answer 30

Combination
Junctional

Question 31

Combinational Diversity

Answer 31

Different combination of gene segments

Question 32

Junctional diversity

Answer 32

Additional nucleotides when recombination occurs

Question 33

Recombination

Answer 33

Joins segments in the correct transcriptional orientation

Question 34

Generating junctional diversity

Answer 34

RSS brought together
RAG complex nicks + open DNA palindromic nucleotides are added
N nucleotides added by Tdt
Unpaired nucleotides excised + DNA repaired