Automation

Question 1

What is directly measured on the Coulter LH?

Answer 1

RBC, Hgb, WBC, diff %

Question 2

What is derived on the Coulter LH?

Answer 2

MCV, RDW, MPV, Plt

Question 3

What is calculated on the Coulter LH?

Answer 3

Hct, MCH, MCHC, Diff absolute value

Question 4

What does ++++ mean on Plt count?

Answer 4

platelet count >999

Question 5

What does ++++ backlit WBC mean?

Answer 5

WBC > 100,000/cumm

dilute and rerun (multiply by dilution)

Question 6

What are the 4 technologies used in SYSMEX series?

Answer 6

impedance
radio frequency
absorp. spec.
flow cytometry

Question 7

On the SYSMEX what method is used to determine Hgb concentration?

Answer 7

SLS (sodium lauryl sulfate)
Hgb converted to methgb
measured @ 555nm

Question 8

What causes a MCHC >37.0 g/dL?

What should be done?

Answer 8

spherocytes (verify w/ smear)
lipemia/icterus (saline replacement/plasma blank for Hgb)
instrument malfunction

Question 9

Where would platelet clumps appear on a histogram?

Answer 9

in the WBC area

Question 10

Where would RBC fragments appear on a histogram?

Answer 10

platelet area

Question 11

What effect does lipemia have on automated CBC results?

Answer 11

false increase Hgb
increased MCHC
(do saline replacement or plasma blank)

Question 12

What effect does WBC >100,000/cumm have on automated instruments?

Answer 12

++++ on printout

dilute and rerun (multiply by dilution)