aubf 2 Flashcards
Urine is coagulated by heat and
precipitated by acetic acid (5-10%) and the degree
of turbidity produced is proportional to the amount
of protein present
Heat and Acetic Acid Test
SULFOSALICYLIC ACID (SSA)/COLD PROTEIN
PRECIPITATION
Reagent:
3% SSa
SULFOSALICYLIC ACID (SSA)/COLD PROTEIN
PRECIPITATION PRINCIPLE:
Most proteins are precipitated by dilute SSA
_=radiographic dyes, tolbutamide
metabolites, cephalosporins, penicillinsand sulfonamides
false +
=highly alkaline urine, very dilute
samples
false-
(+) SSA & (+) RgtStrip=
albumin
(+) SSA & (-) RgtStrip=
proteins other than albumin
(-) SSA & (+) RgtStrip=_
albumin
No increase in turbidity
NEGATIVE
Noticeable turbidity
Trace
Distinct turbidity with
no granulation
1+
Turbidity with
granulation, NO
flocculation
2+
Turbidity with
granulation and
flocculation
3+
Clumps of protein
4+
contains SSA, Sodium sulfate,
Bromphenol Blue
extons reagent
precipitates far more
greater than albumin
Trichloroacetic acid
OTHER TEST FOR PROTEINS IN URINE
HELLER’SRING TEST
HEAT PRECIPITATION TEST
PANDY’S TEST
ESBACH’S TEST
principle of glucose
double sequential enzymatic reaction
reagent of glucose
glucose oxidase peroxidase
Most frequent chemical analysis performed in urine and
synovial fluid detection of:
glucose
_presence of any sugar (reducing or nonreducing in urine).
mellituria
_presence of any reducing sugar in urine
specimen (glucose, lactose, galactose, fructose and
pentose)
glucosuria
HYPERGLYCEMIA
ASSOCIATED
increased in urine increased in blood
RENAL ASSOCIATED
increase urine N blood
provides a specific test for
glucose
glucose oxidase procedure
CLINISTIX
o-toluidine
pinkto purple
MULTISTIX
potassium iodide=
blue to brown
green to brown
CHEMSTRIP
aminopropyl-
yellow to brown
chemstrip Tetramethylbenzidine
yellow to green
general test for glucose an other
reducing sugar
BENEDICTS TEST
relies on the ability of the glucose and
other reducing substances to reduce copper sulfate
to cuprous oxide in the presence of alkali and heat
BENEDICTS TEST
TESTS FOR GLUCOSE
BENEDICTS TEST
COPPER REDUCTION TEST/CLINITEST TABLET
nonspecific for glucose, detects all reducing sugars except
for sucrose
COPPER REDUCTION TEST/CLINITEST TABLET-
