aubf 2 Flashcards
Urine is coagulated by heat and
precipitated by acetic acid (5-10%) and the degree
of turbidity produced is proportional to the amount
of protein present
Heat and Acetic Acid Test
SULFOSALICYLIC ACID (SSA)/COLD PROTEIN
PRECIPITATION
Reagent:
3% SSa
SULFOSALICYLIC ACID (SSA)/COLD PROTEIN
PRECIPITATION PRINCIPLE:
Most proteins are precipitated by dilute SSA
_=radiographic dyes, tolbutamide
metabolites, cephalosporins, penicillinsand sulfonamides
false +
=highly alkaline urine, very dilute
samples
false-
(+) SSA & (+) RgtStrip=
albumin
(+) SSA & (-) RgtStrip=
proteins other than albumin
(-) SSA & (+) RgtStrip=_
albumin
No increase in turbidity
NEGATIVE
Noticeable turbidity
Trace
Distinct turbidity with
no granulation
1+
Turbidity with
granulation, NO
flocculation
2+
Turbidity with
granulation and
flocculation
3+
Clumps of protein
4+
contains SSA, Sodium sulfate,
Bromphenol Blue
extons reagent