assay development

Question 1

define accuracy

Answer 1

how close the measured value and true value.It will determine validity of data

Question 2

what is validation

Answer 2

activity to verify the method use suitable for intended application

Question 3

main criteria for validation

Answer 3

reproducibility,repeatibility,precision

accurancy

sensitivity to lod and loq

Question 4

what is assay

Answer 4

the testing or measurement technique

method or procedure

Question 5

what cause drug decomposition

Answer 5

1. hydrolysis
2. oxidation
3. photolysis

Question 6

describe hydrolysis

Answer 6

due to presence of oxygen
use air tight container
eg:ester, amide, lactams

Question 7

oxidation

Answer 7

due to presence of oxidation agent
presence of trace element as catalyst
can be prevented by using antioxidant
eg;vitamin c,vitamin a, morphine,tetracycline

Question 8

photolysis

Answer 8

due to electromagnectic radiation mainly uv
prevented by suitable packaging &antioxidant
eg:vitamin C,folic acid,riboflavin

Question 9

drug cecomposition product

Answer 9

depend on drug and way of decomposed

Question 10

drug decomposition assay

Answer 10

1. visual test

2. chemical/physical test