Aseptic Techniques in Surgery Flashcards
Why is aseptic technique important?
- surgical disruption of dermal integrity
- introduction of bacteria into wound
- -> endogenous: within/on patient
- -> exogenous: airbone, surgical team
Sterilization
Complete elimination of microbial viability
- bacteria, viruses, spores
Disinfection
Destruction of MOST pathogenic organisms on inanimate objects
Antisepsis
Prevent growth of microorganisms on living tissue by inhibiting activity or killing them (not sterilized)
Living organism too small to be seen with the naked eye
Microbe/microorganism
Microbe capable of causing disease
Pathogen
Detrimental result of invasion by pathogens
Infection
Infection acquired during hospitalization or during attendance at any vet facility
Nosocomial
Methods of sterilization
- steam
- chemical
- plasma
- ionizing
- cold chemical
Steam and pressure
Most common
- destroys bacteria by coagulation and cellular protein denaturation
- load on edge –> no stacking (increased thickness decreases steam penetration
- small amount of air between packs
- gravity displacement: air is heavier than steam
Chemical (gas)
Ethylene Oxide
- flammable, explosive liquid if not mixed with CO2 or freon
- kills via alkylation
- lower temp (120-140 F) for cameras, plastics, cables
- environmental/safety hazards
Plasma
Uses reactive ion, electrons, and other atomic particles to sterilize
- lower temp
- used with vapor phase H2O2
- takes only 75 min, immediate use
- irritant: housed in sealed cassette
Radiation
Ionizing radiation (Cobalt 60)
- used in commercial manufacturing due to expense
- suture material, gowns, drapes, etc
Cold chemical
Glutaraldehyde (2%)
- noncorrosive
- good for delicate lensed instruments
- other: alcohols, chlorhexadine, iodines, phenols, quart, ammonium compounds
Rules for sterilization
- know activity of agent
- know potential toxicities
- properly wrap equipment
- check technique/instruments
- use indicators
- know shelf lives