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Bacteriology/ Mycology > Antimicrobial Resistance > Flashcards

Antimicrobial Resistance Flashcards

1
Q

What are the three major purposes of antimicrobial prescription?

A

Therapeutic: If an individual is sick, treat patient to cure.
Metaphylactics: If one patient is noticed in a heard, treat the whole heard to prevent spread.
Prophylactic: Seasonal prescription of drug to prevent the most susceptible population within a heard.

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2
Q

What is contributing to antimicrobial resistance/ overuse?

A

Metaphylactics: If one patient is noticed in a heard, treat the whole heard to prevent spread.
Prophylactic: Seasonal prescription of drug to prevent the most susceptible population within a heard.
- Antimicrobial overuse in intensive farms (pigs & poultry), dry cow therapy, and shipping of animals

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3
Q

What is the main starting cause of Antimicrobial resistance?

A

Unregulated use of antimicrobials in
animal or humans to AMR bacteria

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4
Q

What is the trend of spread of antimicrobial resistance?

A

Unregulated antimicrobial use -> Use of feces/ water contained with AMR bacteria as fertilizer-> food containing AMR bacteria from feces -> AMR bacteria spread to public via food and water

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5
Q

What is the current morbidity and mortality due to antimicrobial resistance cases?

A

Morbidity : 2 million people in the US
Mortality: 1-27 million people globally

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6
Q

What is the projected morbidity and global economic cost due to antimicrobial resistance cases in 2050?

A

Morbidity : 10 million people in the US annually
Economic cost: 100 trillion

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7
Q

What are the mode of bacterial growth?

A

Plankton life stage finds a place to attach, and begins immobile/ sessile growth. Its biofilm matures and at a certain point it will begin to disperse.

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8
Q

What are the 5 major groups of bacteria? What are they classified by?

A

Bacteria are classified into 5 major groups by their cell wall architecture

  • Classic Gram +
  • Mycobacterium
  • Classic Gram -
  • Chlamydia
  • Mycoplasma
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9
Q

What is a classic gram positive bacteria cell wall architecture?

A
  • several layers ( tiers of peptidoglycan with lipo/teichoic acid.
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10
Q

What is the cell wall architecture of a mycobacterium?

A

peptidoglycan is covered by mycolic acid (wax or lipids)

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11
Q

What is the cell wall architecture of a classic gram negative bacteria?

A

Outer membrane (OM) made up of protiens & lipopolysaccharide ( lipid A & sugar or endotoxin)

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12
Q

What is the cell wall architecture of Chlamydia bacteria?

A

cell wall without peptidoglycan. Outermembrane with protiens and lipopolysaccharide ( lipid A + sugar or endotoxin)

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13
Q

What is the cell wall architecture of mycoplasma bacteria?

A

Mycoplasma has no cell wall

has sterols in cell membrane

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14
Q

Is mycoplasma stained by gram stain?

A

NO

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15
Q

If you have an antimicrobial that acts by inhibiting cell wall, which major group of bacteria will be resistant?

A

Mycoplasma

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16
Q

What are the steps to gram staining bacterial slides?

A
  1. ) Fix bacteria to slide using flame 2-3 x
  2. ) Add crystal violet and aggitate for 60 seconds
  3. ) Add iodine and aggitate for 60 seconds
  4. ) Decolorize slide with (alcohol) for 15 seconds
  5. ) Stain with Safranin for 60 seconds.
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17
Q

What does each letter indicate on this image of a bacteria?

A

A.) Capsule

B.) Porins

C.) Pili

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18
Q

What are biofilms?

A

Dense bacteria community

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19
Q

What are the 6 mechanisms of action of microbials?

A

Cell wall synthesis

Metabolism – folic acid synthesis

30S – protein synthesis

50S – protein synthesis

RNA polymerase – mRNA (RNA synthesis)

DNA gyrase/topoisomerase

(DNA synthesis)

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20
Q

Which antibiotics interfere with bacterial cell wall synthesis?

A

β-lactams

Penicillins

Cephalosporins

Carbapenems

Monobactams

mnemonic: Bitter People Can Carry Misery

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21
Q

Which antibiotics interfere with bacterial metabolism/ folic acid synthesis?

A

Sulfonamides Trimethoprim

Mnemic Second Trimester

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22
Q

Which antibiotics interfere with 30 S bacterial protein synthesis?

A

Tetracyclines

Aminoglycosides

mnemonic: 30S Alarm Tone

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23
Q

Which antibiotics interfere with 50 S bacterial protein synthesis?

A

Mycolides

Chloroamphenicol

mnemonics: 50$ Cash Money

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24
Q

Which antibiotics interfere with RNA polymerase - RNA synthesis?

A

Rifamycins

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25
Q

Which antibiotics interfere with DNA gyrase/topoisomerase (DNA synthesis)?

A

Flouroquinolones

Nitroimidazoles

mnemonic: DNA’s For Nerds

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26
Q

What are the 4 major new AMR mechanisms?

A
  1. ) Reduced permeability
  2. ) Efflux Pumping (vomiting)
  3. ) Drug inactivation by enzymes
  4. ) Target site change, modification or protection.
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27
Q

How does Biofilm formation also affect AMR?

A
  1. ) Induced extracellular polymer (matrix) production as a barrier
  2. ) Thick impenetrable communities as a barrier
  3. ) Low nutrient & oxygen supply to the center
  4. ) Dormant spore-like cell in the center for persistence

Limit uptake due to low permeability

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28
Q

What antibiotics are less efficient due to the following AMR mechanism: Mutation on the porin channel to reduce membrane permeability & limit entry of the drug into cytoplasm

A

Amonoglycosides

Quinolones

beta-lactam

Chloramphenicol

Rifamycin

Tetracycline

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29
Q

What antibiotics are less efficient due to the following AMR mechanism: Acquire efflux transporter genes or increase their expression for pumping out the drug from the cytoplasm

A

Tetracycline

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30
Q

What antibiotics are less efficient due to the following AMR mechanism: Change the drug target sites by modification, mutation, capping or protection of binding-site

A

Amonoglycosides

Tetracycline

Rifamycin

Quinolones

beta-lactams

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31
Q

What antibiotics are less efficient due to the following AMR mechanism: Expression of potent enzymes that destroy or enzymatically modify the drug (mainily encoded by plasmid)

A

beta-lactams

Amonoglycosides

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32
Q

What is the major AMR mechanism that inhibits Beta lactams and amonoglycosides?

A

Expression of potent enzymes that destroy or enzymatically modify the drug (mainily encoded by plasmid)

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33
Q

What is the major AMR mechanism that inhibits Chloramphenicol Microlids?

A

Change the drug target sites by modification, mutation, capping or protection of binding-site

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34
Q

What is the major AMR mechanism that inhibits Quinolones?

A

Change the drug target sites by modification, mutation, capping or protection of binding-site

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35
Q

What is the major AMR mechanism that inhibits Rifamycin?

A

Change the drug target sites by modification, mutation, capping or protection of binding-site

36
Q

What is the major AMR mechanism that inhibits tetracycline?

A

Acquire efflux transporter genes or increase their expression for pumping out the drug from the cytoplasm

37
Q

What antimicrobial resistance mechanism is used against betalactams in gram negative bacteria?

A

Gram negative (uptake ESBL &/or carba plasmids): beta-lactamase enzyme production

38
Q

What antimicrobial resistance mechanism is used against betalactams in gram positive bacteria?

A

Gram positive (uptake SCC) : Change in binding site of beta-lactams (mutation of peptidase /PBP)

39
Q

What are the ESBL enzymes that destroy beta lactam antimicrobials that are transmitted by plasmids?

A

TEM = named for patient Temoneira

SHV = sulfhydryl variant enzyme

CTX-M = cefotaximase

40
Q

What are the carbapebenase enzymes that destroy beta lactam antimicrobials that are transmitted by plasmids?

A

KPC (Klebsiella pneumoniae carbapenemase)

IMP (Imipenemase metallo-beta-lactamase)

NDM (New Delhi metallo-beta-lactamase)

VIM (Verona integron-encoded metallo-beta-L)

OXA (Oxacillin carbapenemases)

41
Q

What caused the origin of AMR?

A
  • Acquired resistance- > horizontal gene transfer -> 1.) Dna in nature (transformation). 2.) Plasmid (conjugation) 3.) Bacteriophage (transduction)
  • Intrinsic (innate/ natual resistance -> Vertical gene transfer
42
Q

What antimicrobials do not work on mycoplasma and why?

A

These bacteria are resistant to antimicrobials that act on the cell wall. These are examples of those bacteria:

Penicillin

Cephalosporin

Carbapenem

Monobactum

43
Q

What are the 3 sets of guidelines for AMR detection that we should follow if needed?

A

Clinical & Laboratory Standards Institute (CLSI), USA

European Committee on Antimicrobial Susceptibility Testing (EUCAST) or

International Standardization Organization (ISO)

44
Q

What are examples of enterobacteriaceae targeted for AMR testing?

A
  • E.coli
  • Salmonella
  • Klebseilla
  • Citrobacter
  • Enterobacter
  • Proteus
45
Q

What are examples of other gram negative bacilli targeted for AMR testing?

A

Acinetobacter

Pseudomonas

46
Q

What are examples of non fastidious gram positives targeted for AMR testing?

A

Staphylococcus

47
Q

What are examples of fastidious organisms targeted for AMR testing?

A

Streptococci

Campylobacter,

Erysipelothrix,

Listeria,

Hemophilus,

Neisseria,

Helicobacter

48
Q

What are examples of Bioterrorism agents targeted for AMR testing?

A

B. anthracis,

Yersinia pests,

Brucella,

Franci sella tularensis

Burkholderia mallei

peudomallei

49
Q

What are examples of anerobic microbes targeted for AMR testing?

A

Fusobacterium

Clostridium,

Bacteroides,

50
Q

What are the 6 bacteria species termed as ESKAPE which are famous in AMR globally?

A

Enterococcus

S. aureus

K. pneumoniae

A. baumannii

P. aeruginosa

Enterobacter

51
Q

What bacteria is cultured on mannitol salt agar?

A

Staphylococcus,

Enterococcus,

Listeria

Micrococcaceae

52
Q

What bacteria is cultured on edward media?

A

Streptococcus

Enterococcus

53
Q

What bacteria is cultured on kenner-fecal agar media?

A

Enterococcus (selective media)

54
Q

What bacteria is cultured on MacConkey agar?

A

Enterobacteriaceae

Enterococcus

55
Q

What is the type of broth media used for Aerobic bacteria to screen isolates for AMR?

A

Cation-adjusted Mueller-Hinton broth

56
Q

What is the type of agar media used for Aerobic bacteria to screen isolates for AMR?

A

Cation-adjusted Mueller-Hinton agar

57
Q

What is the type of broth media used for Anaerobic bacteria to screen isolates for AMR?

A

Brucella broth + (5µg/ml hemin, µg/ml vitamin K and 5% lysed horse blood)

58
Q

What is the type of agar media used for Anaerobic bacteria to screen isolates for AMR?

A

Brucella agar +3-5% lysed horse blood

59
Q

What is disc diffusion? Is it a qualatative or quantitative method?

A

This is when bacterial cultures are grown on an agar disc, but discs of various antibiotics are placed to determine the sensitivities to specific antibiotics by the grown culture. This is a qualatative method

60
Q

What is an E test? Is it a qualatative or quantitative method?

A

Culture is grown on a plate and strip impregnated with a drug is placed on a solid media. the strip has decreasing quantities of drug placed as you move towards the center so this will show the minimum inhibitory concentration. This is quantitative method.

61
Q

What test type is seen in this image?

A

E test

62
Q

What test is seen in this image?

A

Disc diffusion

63
Q

What is agar dilution? Is this Quantitative or Qualitative? What will this method give you in terms of results

A

Agar dilution involves the incorporation of different concentrations of the antimicrobial substance into an agar medium followed by the application of a standardized number of cells to the surface of the agar plate.

Quantitative method

MIC: Minimum inhibitory concentration

64
Q

What is macrodilution? Is it quantitative or qualatative?

A

After a culture is done, the colonies are placed in rich media broth and left overnight. The colonies are then added equally to a series of dilutions of antimicrobials. You also will plate your control sample to make sure bacteria is still viable. All of these samples will be left overnight. The next day you will see the MIC is the tube that is the lowest without bacterial growth.

65
Q

What would the MIC be in this example of macrodilution?

A

8 ug/ml

66
Q

What test is seen here?

A

microdilution

67
Q

What is a microdilution?

A

(1) first prepare 2-fold serial dilutions of the test compounds (up to 7) and one quality control (QC) antibiotic in a microdilution plate.
(2) Create the inoculum by taking a few colonies from an agar plate with a sterile swab, preparing a McFarland standard, and diluting the McFarland standard into media.
(3) Dispense the inoculum into the microdilution plate with the serial diluted test compounds and incubate the microdilution plate.
(4) Read the microdilution plate to determine the MIC value.
(5) Plate a portion of each well on an appropriate agar media, incubate the agar, and check for colonies to determine the MBC.

68
Q

What are the quality controls used for a survey on beta lactamases producing bacteria?

A

E.coli ATCC 35218

Klebsiella pneumoniae ATCC 700603

Klebsiella pneumoniae ATCC BAA-1705 or BAA-2814

Acinectobacter baumannii NCTC 13304

69
Q

What are the quality controls used for a survey on non- fastidious bacteria for agar/ broth dilution?

A

E.coli ATCC 25922

Pseudomona aeruginosa ATCC 27853

Enterobacter faecalis ATCC 259212

Staphylococcus aureus ATCC 29213

70
Q

What are the quality controls used for a survey bacteria of bioterrorism?

A

E.coli ATCC 25922

Staphylococcus aureus ATCC 29213

71
Q

When would you invalidate a test after using known reference bacterial strains?

A

Invalidate the test if:

  • there is no viable & pure growth of the quality control strains

or

  • their cut-off value inhibition zone by disk or MIC is not within the range defined by CLSI, EUCAST or ISO
72
Q

What is the list of 11 antimicrobial classes ?

A
  1. )Aminoglycosides. e.g. Amikacin, Gentamycin, Kanamycin, Neomycin, Streptomycin
  2. ) Carbapenem. e.g. Ertapenem, Imipenem, Meropenem
  3. ) Cephalosporins (G1-G5). e.g. Cephalothin, Cefoxitin, Cefotaxime, Ceftazidine, Ceftiofur, Cefepime, Ceftaroline.
  4. ) Chloramphenicol. e.g. Florfenicol
  5. ) Lincosamide e.g. Pirlimycin.
  6. ) Microlides. e.g. Erythromycin, Spiramycin.
  7. ) Penicillins. e.g. Ampicillin,Amoxicillin, Methicillin, Oxacilli.
  8. ) Polypeptides. e.g. Polymyxin B, Bactracin, Colistin.
  9. ) Quinolones (G1-G4). e.g. Nalidixic acid, Ciprofloxacin, Norfloxacin, Moxifloxacin
  10. ) Sulfonamides. e.g. Sulfadimethoxine, Trimethoprim-sulfamethoxazone.
  11. ) Tetracycline: e.g. Oxytetracycline

Any Cat Could Carry Large Mice Probably. Physiologic Quickness Stops Them. ( IDK what else to make, keep thinking ***

73
Q

What is a general rule when setting up an agar/ broth dilution?

A

General rule: 1. The agar/broth dilution should include the drug concentration levels below the susceptible & above the resistant breakpoints of the CLSI guidelines.

Test against few number of antimicrobials that you use in your state or

farm/clinic because you cannot do the AMR testing for all of the antimicrobials on the market

74
Q

Waht is the MIC Cut off values for most antimicrobials?

A

E.g. MIC cut-off values of most antimicrobials ranges between

0.5 to 64 μg/ml

75
Q

What test is not reccomended fo anerobic bacterial cultures?

A

Disc Diffusion

76
Q

What media should be used on anerobic bacteria? (Actinomyces, Bacteroides, Clostridium, Fusobacterium)

A

Use Brucella agar or broth media

77
Q

What media is ideal for fastidious aerobic bacteria? (Campylobacter, Erysipelothrix, Haemophilus, Helicobacter, Listeria, Neisseria, Pasteurella, and Streptococcus)

A

Cation-adjusted Mueller-Hinton broth or agar

Disc diffusion or E test

78
Q

What media is ideal nonfastidious fast growing aerobic bacteria in 16-20h? (Staphylococcus, Entrobacteriaceae, pseudomona)

A

Cation-adjusted Mueller-Hinton broth or agar

Disc diffusion or E test

79
Q

How can you control the fast spreading AMR on farms, national and worldwide?

A
  • Survaillence
  • Education
  • Alternatives to antimicrobials
80
Q

What is the benefits of surveillance of AMR? (i.e periodic survey to measure in the population, food, feed, water, etc.)

A
  • Survey on drug use pattern and prioritize the different drugs for use
  • Develop and use faster diagnostic testing of AMR
  • Survey regularly antimicrobial resistance patterns in pathogens
81
Q

What are the benefits of education of AMR?

A

Educate expertise for reducing drug use in animals & regulate, monitor and evaluate if the drug use is as per the set priority

  • Therapeutic use (only treat the sick individuals with required dose, duration and frequency)
  • Educate against Metaphylactic use and inform of harm caused. (if one sick individual is observed, treat the whole herd/flock policy)
  • Advise against : Prophylactic use (blanket seasonal use even if there is no sick animal)
82
Q

What are the ways we can find alternatives to antimicrobials for animals?

A
  • Improved animal hubandry and nutrition ( less stress, immune system can function better)
  • Use vaccines and invest on new vaccine development
  • New antimicrobial discoverys
  • Phage therapy
83
Q

What 4 main mechanisms do bacteria use for AMR?

A

porin, binding site, enzyme, and pumping

84
Q

Where can bacteria aquire AMR genes from?

A

Bacteria can acquire AMR genes from at least 4 sources: plasmid, phage, free DNA, & parents

85
Q

What are the 5 culture based assays that can be used to test isolates for AMR?

A
  • Testing of isolates for AMR can be done by 5 culture based assays: Disc diffusion, E-test, agar dilution, macrodilution, and microdilution
  • PCR, whole genome sequencing, serology can also be used to detect AMR
86
Q

What are 6 broad interventions that can control AMR?

A

AMR may be controlled by 6 broad interventions: judicious drug use, surveillance, diagnosis, discoveries, collaborations, & policy/practice change

Bacteriology/ Mycology (19 decks)

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