Antigen receptor Diversity L3 Flashcards
What does the Naive B cell consist of in terms of Ig?
IgM and IgD
What does “Naive” B cell mean?
It has not been in contact with an Ag
T/F: IgM and IgD do not have ths ame specificity
FALSE
They do have the same specificty
What does the light chain consist of?
VJC (C=constant region)
What does the Heavy chain consist of?
VDJC
What does VDJ recombination require?
RAG-1 and RAG-2 proteins
Where are RAG 1 & RAG 2 expressed?
In both B and T cells
What happens of RAG 1 or RAG 2 or both are absent or defective?
NO ACQUIRED IMMUNITY
What is the Magnitude of VDJ?
V > D > J
Name the 5 Generation Ab diversity?
- Germline
- Combinational
- Junctional
- Somatic Hypermutation
T/F: high affinity is crucial for Ab system
TRUE
What is Class Switching Recombination?
Using different constant regions
T/F Class Switching Recombination is disctint from VDJ recombination
TRUE
What is Somatic Hypermutation?
Point mutations occuring in variable regions of heavy and ligh chains
Where does CSR and SHM occur?
In germinal centers of the secondary lymphoid organs
What does RAG 1 and RAG 2 do?
Mediate the rearrangement of Ig genes in both B and T cells (developing)
What are IgM and IgD in the naive B cell have ths ame specificity?
They are using the same variable region
What is combinational diversity in VDJ/VDJC?
Rearrangement of the letters to give you different outcomes (low millions)
Where does the majority of the diversity of VDJ/VDJC come from?
Junctional and SHM
What physically happens to you when CSR happens?
Swollen Lymph nodes
