animal models-2

Question 1

what question are you trying to answer when you do the conditioned place preference

Answer 1

does my new drug have abuse potential?

Question 2

what maze is good for an initial test of memory and function

Answer 2

the Y-maze

Question 3

what is the Y maze sensitive to (what 3 things can it test for)

Answer 3

• hippocampal damage
• gene manipulation
• amnestic drugs

Question 4

what kind of test is good to know if the drug is rewarding or not

Answer 4

the conditioned place preference test

Question 5

how many chambers in the conditioned place preference test

Answer 5

2

Question 6

where will the animal go if given a CNS stimulant like cocaine in a conditioned place preference test

Answer 6

to the environment associated with the repeated administration

Question 7

what are photocells used for in conditioned place preference test

Answer 7

they allow for automatation (they are in both chambers so maybe help with some consistency idk)

Question 8

how can you compare the abuse potential of 2 drugs using conditioned place preference test

Answer 8

train rat with 1 drug, then substitute with another drug and see if it can substitute it well for the animal

Question 9

what does the conditioned place preference test measure + where in the brain

Answer 9

positive reinforcing properties of a drug, actions that are at the level of the mesolimbic dopamine system

Question 10

what are 4 drugs that cause place preference

Answer 10

nicotine
PCP
cocaine
diazepam

Question 11

what kind of chamber is used for drug discrimination tests

Answer 11

operant chamber, like a skinner box

Question 12

what signals the start of a drug discrimination session

Answer 12

red or green light

Question 13

what are rats trained to do in drug discrimination (and in response to wait)

Answer 13

trained to press a lever in response to light (example, press right lever with green light)

Question 14

what happens if the rat pressed the lever with light correctly

Answer 14

they get food pellet, reward

Question 15

what do you do in the drug discrimination test after you established that light=lever=reward

Answer 15

inject with vehicle or drug before placing it in the chamber

Question 16

does the saline or drug correspond to green light sessions

Answer 16

drug

Question 17

does the saline or drug correspond to red light sessions

Answer 17

saline

Question 18

in the drug discrimination tests, what are you pairing an event with (2 things, what first then after)

Answer 18

a reward, then drug or saline

Question 19

what happens once the animal knows to press the right or left lever when given either drug or saline? (Whats the next step)

Answer 19

remove the light to see if the animal will press the lever with just drug input

Question 20

how will you know if an animal thinks 2 drugs are the same in a drug discrimination trial

Answer 20

a drug that the rat perceives to be the same will result in right lever presses

Question 21

does the drug have to be addictive to work in drug discrimination tests

Answer 21

no

Question 22

what is required for a drug to pass as being similar in a drug discrimination test (even if its not addictive)

Answer 22

it has to generate a response that is different from your regular state

Question 23

what is a drug detection procedure

Answer 23

the process of drug discrimination trail test thing

Question 24

what is the elevated plus-maze / y-maze based on

Answer 24

the fact that rodents would rather be in the enclosed arms than in the open arms

Question 25

what do they score/measure in the elevated-plus maze

Answer 25

the entries and time spent in the open arms vs closed arms

Question 26

which arms in the elevated-plus maze gives more anxiety to rats

Answer 26

when they are in the open arms

Question 27

what does anti anxiety drugs in the elevated-plus maze do to rat

Answer 27

increases the time in the open arms

Question 28

do benzodiazepines work in the elevated-plus maze paradigm

Answer 28

yes

Question 29

does buspirone work in the elevated-plus maze paradigm

Answer 29

no

Question 30

which drug is expected to work in the elevated-plus maze but doesnt work

Answer 30

buspirone

Question 31

what is mechanism of action of the buspiron

Answer 31

5HT1A antagonist

Question 32

what is the vogel test (what happens in the procedure)

Answer 32

you punish drinking water with mild electrical shocks, then see how drugs can change their behaviour

Question 33

what is the reason for performing the vogel test

Answer 33

fast screening of the potential anxiolytic properties of drugs

Question 34

what reestablishes drinking responses in the vogel test

Answer 34

drugs with anxiolytic properties

Question 35

does buspirone work in the vogel shock test

Answer 35

no

Question 36

what does the water maze test for

Answer 36

learning and cognition

Question 37

how is the water maze set up

Answer 37

large circular pool of water with a hidden platform, visual cues help them find it again

Question 38

how many trials in the water maze is usually required to get to the platform quiz

Answer 38

2-3

Question 39

how can we use the morris water maze to test for drugs and disease

Answer 39

see what can affect memory and learning, like benzos or cognitive enhancers

Question 40

can brain damage inhibit the water maze results (and where is the damage important)

Answer 40

yes, mostly in the hippocampus

Question 41

what are 2 treamtments for alzheimers

Answer 41

cholinesterase inhibitors and memantine

Question 42

why is cholinesterase inhibitors used for alzheimers

Answer 42

they are designed to prevent the breakdown of ACh which is important for memory and thinking skills

Question 43

why is memantine used for alzheimers

Answer 43

it regulates glutamate which serves to process, store and retrieve info

Question 44

what was the point of using the radial arm mase

Answer 44

measure spatial memory