Anatomy - Pneumonia Flashcards
What is volume of distribution?
Apparent volume in which drug is dissolved in body = amount in body / conc. = dose / conc. at t=0
What is clearance?
Volume of plasma cleared of drug in unit time (ml/min or L/h) = renal + hepatic clearance (eGFR)
What does a first order elimination graph look like?
What is the equation?
Rate of elimination = proportional to drug concentration
Ct = C0e-kt
Ct = conc. at t=t
C0 = conc. at t=0
k = rate constant (per minute or per hour)
t = time
What does the rate constant (k) stand for?
Fraction eliminated per unit time = clearance / Vd
How do you work out half-life using equation?
t1/2 = loge2 / k
k = loge2 / t1/2
What are the properties of IV infusion?
Administration of drug at constant rate (0 order)
Steady state = elimination (first order graph plateau) = infusion rate
Input = output = Css
Maintenance dose = amount removed Infusion rate = clearance X Css
What is aminophylline and its properties?
- Used for severe asthma
- Bronchodilator
- Given intravenously
- Is salt of theophylline (80% of aminophylline is theophylline)
- Infusion rate = (CL X Css) / 0.8
What does and oral dosing graph look like and its properties?
- Curve = influenced by rate of dissolution and rate of absorption
- Rise to peak = absorption + elimination
- After peak = just elimination
- Functional half-life = prolonged absorption super-imposed on elimination (slow oral)
How do you work out bioavailability?
How do you work out dose given?
Bioavailability = F = fraction absorbed = AUC oral / AUC iv
Dose given = amount needed / F
What are the properties of repeated oral dose?
- Each dose is independent of each-other if given far apart enough from each-other
- Aim = to reach stead-state
- Takes 5 half-lives (approx..) to reach steady state
What is the dose calculation equation?
What does ‘tau’ stand for?
What does doubling the dose do?
tau = dosage interval
Double dose = double concentration
What do you need for designing dosage regimens?
- Measure therapeutic window
- Loading dose large initial does to rapidly increase plasma conc. to therapeutic window range = target level X V/F
- Elimination half-life
What are the properties of gentamicin dosaging?
Have period where dose is subtherapeutic and below therapeutic window to reduce damage done to kidneys and ears
Given via IV boluses
+ clearance renally
CL = CLcreatinine
What does the Hartford nomogram for gentamicin llok like and how do you find correct dose?
Use sampling
What is anti-microbial chemotherapy and it’s targets?
The use of drugs to selectively kill bacteria (and viruses / fungi) without affecting the host.
Targets:
- Bacterial cell wall
- Bacterial ribosomes
- Bacterial folate metabolism
- Bacterial DNA gyrase
Antibiotics
- Produced by an organism to attack other organisms eg penicillin
Antibacterials
- Man-made chemicals
What type of antibiotic are penicillins and what are it’s properties?
‘Beta’-lactam antibiotic
- Inject with enzyme penicillin if suspected meningitis
- Beta-lactam ring gives chemistry of drug
- Inhibit bacterial cell wall synthesis à irreversibly bind to a transpeptidase which cross-links peptidoglycans in cell wall
- Only effective against dividing organisms
- Bactericidal, causing bacterial lysis
What are the properties of beta-lactamases?
- Secreted by resistant bacteria
- Deactivates some penicillins
- Other penicillins are resistant to them
- Inhibited by clavulanic acid + other agents –> Co-amoxiclav
What are the properties of a penicillin allergy?
- Penicillins are immunogenic
- Allergic reaction develops upon repeat exposure
What type of antibiotic are cephalosporins and how do they work?
‘Beta’-lactam antibiotics
- Act in similar way to penicillin
- Inhibit transpeptidases which cross-link peptidoglycan chains
- Cross-reactivity - penicillin allergy = cephalosporin allergy in small no. of people
How do glycopeptides work and give an example?
Vancomycin
- Inhibit bacterial cell wall synthesis by inhibiting peptidoglycan chain growth
- Largely bactericidal
- Used for superbug infections
Give and example of a tetracycline and how do they work and properties?
Tetracycline
- Inhibit protein synthesis by binding to 30S subunit of bacterial ribosome
- Prevent tRNA binding to acceptor site (A site)
- Actively accumulate in bacterial cells
- Are bacteriostatic
- Decreased use from resistance increase
Give an example of a macrolides and how do they work and properties?
Erythromycin
- Prevent translocation of 50S subunit of bacterial ribosome along mRNA
- Prevent protein synthesis
- Are bacteriostatic
- Used as penicillin alternative for those with allergies
- Are cytochrome P450 inhibitors and associated with + drug interactions
Give an example of an aminoglycoside and how do they work and properties?
Neomycin
- Irreversibly bind to 30S subunit of bacterial ribosomes, causing misreading of mRNA
- Interfere with protein synthesis
- Are bactericidal
- Used to manage Gram-negative bacteria
- Complicated use due to toxicity
- Have complex regimens
Give an example of a quinolone and how do they work?
Ciprofloxacin
- Inhibit bacterial DNA gyrase (topoisomerase ll) and topoisomerase lV
- Gram-negative bacteria = inhibit supercoiling of bacterial DNA, essential for DNA repair and replication à DNA gyrase is target
- Gram-positive bacteria = interfere with separation of DNA strands during replication à Topoisomerase lV is target
What is trimethoprim and what are it’s properties?
- Structurally related to folate
- Is a folate antagonist
- Inhibits bacterial dihydrofolate reductase which converts folate to tetrahydrofolate
- Less potent against human form of enzyme
- Are bacteriostatic
- Used for UTIs
What are the properties of sulphonamides?
- Analogues of p-aminobenzoic acid (PABA)
- Inhibit growth of bacteria by competitively inhibiting dihydropteroate synthase (involved in folate synthesis from PABA)
- Reduction of DNA and RNA precursors
What are the properties of metronidazole?
- Pro-drug that’s activated by anaerobic bacteria to cytotoxic products
- Damage DNA helical structure, protein and cell membrane
- Used against anaerobic bacteria and protozoa
- Can’t drink alcohol
What are the steps of antibiotic selection?
- Guided by sensitivity of organisms
- Broad spectrum antibiotics
- Resistance
- “Complete the course”
- Allergies
- Pharmacokinetics:
- IV for rapid effects
- Oral route: depends on bioavailability
- Drug interactions
What is the most important antibiotic feature?
Selective toxicity
What are the differences in gram positive and negative bacteria?
What are the properties of an upper respiratory tract infection?
Who is community acquired pneumonia more common in?
Men
Elderly
Alcoholics
Chronic disease sufferers
What are the main symptoms of pneumonia?
- Sputum
- Coughing
- Coughing up blood
What microbes cause community acquired pneumonia?
- Conventional bacteria (60-80%)
- ‘atypical’ bacteria (10-20%) (mycoplasma and legionella)
- Viruses (10-20%)
What are the steps for community acquired pneumonia investigation?
- Confirm diagnosis
- Assess disease severity
- Identify aetiological agent
- Identify somplications
What tests do you do to confirm diagnosis of CAP?
- Temperature
- Full blood count
- U rea, electrolytes and LFT
- CXR
- Arterial por capillary blood gases
What are the microbiological investigations for CAP?
- Sputum culture and sensitivity
- Blood cultures
- Urine for pneumococcal antigen
- Investigation for legionella if suspected
- PCR/immunofluorescence for atypical organisms and viruses
- Pleural fluid for microscopy, culture and antigen detection
What are the 3 steps for CAP management?
- Correction of respiratory failure
- Correction of haemodynamic compromise
- Specific antimicrobial therapy
What tests and values determine the severity of CAP?
- Confusion –> mini-mental test = 8
- Urea –> > 7mmol/l
- Respiratory rate > 30 bpm
- Blood pressure –> sys < 90 mmHg dia < 60 mmHg
- Older than 65
What are the 3 basis for antimicrobial chemotherapy?
- Assessment of likely pathogen
- Severity of illness
- Likelihood of drug resistance
What is the treatment for low severity CAP (score0-1)?
Amoxicillin 500mgs qds / 5 days OR doxycycline 200mgs loading them 100mg od
Unable to take oral therapy = iv amoxicillin 500mgs tds OR iv cefuroxime 1.5g tds if mild penicillin allergy
Sever penicillin allergy = ciprofloxacin 400mgs bd + vancomycin
What is the treatment for moderate severity CAP (score 2)?
Amoxicillin 500mgs – 1g tds 7 days + clarithromycin 500mgs bd for 7 days OR doxycycline 200mgs loading then 100mg od
Unable to take oral therapy = iv amoxicillin or benzylpenicillin (cefuroxime 1.5 g tds if mild penicillin allergy) + iv clarithromycin 500 mgs bd
If severe allergy to penicillin ciprofloxacin 400 mgs bd + vancomycin
What is the treatment severe CAP (score >3)?
Co-amoxiclav 1.2 g tds +clarithromycin 500 mgs bd iv for 10 days
If severe allergy to penicillins or likely MRSA:
Levofloxacin 500 mgs bd + vancomycin 1 g bd
Treat for 10 days - extending to 14-21 days for legionella, staphylococcal or Gram neg pneumonia
What are the properties of hospital acquired pneumonia?
- HAP is defined as pneumonia that occurs more than 48 hrs after hospital admission or within 10 days of a previous admission
- HAP is the second most common infection acquired in hospital
- Candidate organisms include: E.coli, Klebsiella spp., proteus spp., S. pneumoniae, S. aureus (MSSA or MRSA)