Analytical techniques

Question 1

What does HPLC stand for and what is its use?

Answer 1

High performance (pressure) liquid chromatography.
It is used as a method of analysis for the quantification of drugs

Question 2

What is on the x and y axis of HPLC graph?

Answer 2

y - absorbance

x - time

Question 3

How is separation achieved in HPLC?

Answer 3

normal phase

reverse phase

Question 4

HPLC involves mobile phase flowing over stationary phase in a column. True or false?

Answer 4

True

Question 5

What is the stationary phase in HPLC?

Answer 5

Fine silica particles

Question 6

What is the column made from in HPLC?

Answer 6

Stainless steel

Question 7

Reverse phase HPLC is more common in pharmaceutical sciences. Why?

Answer 7

Because most drugs are too polar to be separated using normal phase

Question 8

In normal phase HPLC, the stationary phase is polar and the mobile phase is non-polar. True or false?

Answer 8

True

Question 9

In normal phase, least polar analytes are eluted first, followed by increasing polarity. True or false?

Answer 9

True

Question 10

The performance of HPLC can be improved by making the column length longer. True or false?

Answer 10

True