Analysis RQP- how paper chromatography is used to separate and distinguish coloured substances Flashcards
1
Q
step 1
A
draw a pencil line across the chromatography paper, 1 - 2 cm from the bottom
2
Q
step 2
A
use a pipette or capillary tube to add small spots of each ink to the line on the paper
3
Q
step 3
A
place the paper into a container with a suitable solvent in the bottom
4
Q
step 4
A
allow the solvent to move through the paper, but remove the
chromatogram
before it reaches the top
5
Q
step 5
A
allow the chromatogram to dry, then measure the distance travelled by each spot and by the solvent
6
Q
step 6
A
calculate (and compare) the Rf value of each spot by using distance travelled by substance divided by the distance travelled by solvent (water)