Analysis RQP- how paper chromatography is used to separate and distinguish coloured substances Flashcards

1
Q

step 1

A

draw a pencil line across the chromatography paper, 1 - 2 cm from the bottom

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2
Q

step 2

A

use a pipette or capillary tube to add small spots of each ink to the line on the paper

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3
Q

step 3

A

place the paper into a container with a suitable solvent in the bottom

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4
Q

step 4

A

allow the solvent to move through the paper, but remove the
chromatogram
before it reaches the top

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5
Q

step 5

A

allow the chromatogram to dry, then measure the distance travelled by each spot and by the solvent

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6
Q

step 6

A

calculate (and compare) the Rf value of each spot by using distance travelled by substance divided by the distance travelled by solvent (water)

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