Analysis Pathways Flashcards
What is forward genetics?
When you have a phenotype and look for the gene that causes it
What is reverse genetics?
When you mutate a gene to find its function and the corresponding phenotype
What type of mutations is complementation testing used for?
Recessive mutations
What does no mutant phenotype in a complementation test mean?
They complement each other
What phenotype is there if the genes do not complement each other and what does it mean about the mutation?
Mutant phenotype
Mutation lies in the same gene
If you cross heterozygotic mutants and 25% of their offspring show mutant phenotype what does the complementation test show?
That the genes do not complement each other and the alleles lie in the same gene
What is an allelic series?
Describe different mutant alleles of a gene that cause a range of phenotypes, whereby each one carries a single point mutation within different regions of the same gene
Which organism is often used for an example of an allelic series and give example of phenotypes
C. elegans
Wild type, hypomorph, amorph
What can complementation testing reveal?
Mutations in different genes that result in similar phenotypes–> the gene products work in the same molecular pathway
What type of mutation in different genes can complementation testing reveal?
Ones that result in similar phenotypes
What are the two genes which control mouse colour?
Kit and Steel
What does Steel code for?
A ligand
Which gene in mice codes a receptor involved in controlling mouse colour?
Kit
What does Kit code for?
A receptor for Steel factor
Which gene in mice codes for a ligand involved in controlling mouse colour?
Steel
