Anaerobic GNCs, GPC, GNRs Flashcards
Name the organism
- Gram Stain
- Colony Morphology
- Normal Flora
- Distinguishing reactions/characteristics
Veillonella spp.
- GNC
- Circular, smooth/creamy, raised
- Oral, GU (GI)
- Nitrate POSITIVE
Peptococcus Niger
- Distinguishing reactions/characteristics
Produces a black to olive green pigment
Name the organism
- Gram Stain
- Colony Morphology
- Normal Flora
- Distinguishing reactions/characteristics
Finegoldia Magna
- GPC
- “Donut appearance”
- GI
- Most pathogenic of the Anaerobic GPCs
Name the organism
- Gram Stain
- Colony Morphology
- Normal Flora
Parvimonas micra
- GPC
- Circular, smooth, raised — white to gray color, slight beta hemolysis
- Oral
Name the organism
- Gram Stain
- Colony Morphology
- Normal Flora
- Distinguishing reactions/characteristics
- GLC pattern
Peptostreptococcus anaerobius
- GPC (elongated)
- Circular, smooth/creamy, raised
- Oral, GI
- SPS susceptible
- a…IC
Objective 8: Recognize an organism that is typically found as a contaminant in blood or CSF cultures
Staphylococcus saccharolyticus
Normal flora of Staphylococcus saccharolyticus and Streptococcus intermedius, respectively
Skin and Oral
Objective 7: Associate disease manifestations with their causative agent. (generally based on where organisms are normal flora)
- Streptococcus intermedius
Brain abscess
Name the organism
- Gram Stain
- Colony Morphology
- Normal Flora
- Distinguishing reactions/characteristics
- GLC pattern
Fusobacterium necrophorum
- GNR - short and long
- “Fried egg” appearance
- Oral
- Lemierre’s syndrome, brain abscess
- aB
Name the organism
- Gram Stain
- Colony Morphology
- Normal Flora
- Distinguishing reactions/characteristics
- GLC pattern
Fusobacterium nucleatum
- GNR, spindle-shaped with pointed/tapered ends
- “CIS” or “Bread crumbs” appearance (not seen)
- Oral
- Indole POSITIVE
- aB
Objective 7: Associate disease manifestations with their causative agent. (generally based on where organisms are normal flora)
- Fusobacterium necrophorum
Lemierre’s syndrome, brain abscess
Name the organism
- Gram Stain
- Colony Morphology
- Normal Flora
- Distinguishing reactions/characteristics
- GLC pattern
Fusobacterium mortiferum
- GNR, “Spaghetti and meatballs” appearance (from broth)
- “Whirl” appearance
- GI
- Resistant to antibiotics
- aB
Name the organism
- Gram Stain
- Colony Morphology
- Normal Flora
- Distinguishing reactions/characteristics
- GLC pattern
Bacteroides fragilis
- GNR (vacuoles may be present in broth)
- “CIS” appearance
- GI
- Stimulated by bile, pencillinase producer - resistant to antibiotics, Indole
NEGATIVE - ap (ib) iv
Name the organism
- Gram Stain
- Colony Morphology
- Normal Flora
- Distinguishing reactions/characteristics
- GLC pattern
Bacteroides ovatus
- GNR- egg shaped (vacuoles may be present in broth)
- Wet, mucoid
- GI
- Stimulated by bile, pencillinase producer - resistant to antibiotics, Indole
POSITIVE - ap (ib) iv
Bacteroides thetaiotaomicron
- Gram Stain
- Colony Morphology
- Normal Flora
- Distinguishing reactions/characteristics
- GLC pattern
- GNR, “greek letters” (vacuoles may be present in broth)
- Wet, mucoid
- GI
- Stimulated by bile, pencillinase producer - resistant to antibiotics, Indole
POSITIVE - ap (ib) iv
Objective 6: Recognize the test that allows the B. fragilis group to be divided into two groups
Indole
Porphyromonas spp.
- Distinguishing reactions/characteristics
- Normal flora
- Produces buff/tan-black pigment; brick red fluorescence with UV light
- Mouth
Name the organism
- Gram Stain
- Colony Morphology
- Distinguishing reactions/characteristics
Prevotella melaninogenica
- GNR
- yellow to brown/black colonies
- produces pigment that causes colonies to become black/brown, brick red
fluorescence with UV light, beta lactamase production
Normal flora
- Prevotella oralis
- Prevotella bivia
- Prevotella disiens
- Mouth
- GU
- GU
Name the organism
- Gram Stain
- Colony Morphology
- Distinguishing reactions/characteristics
Campylobacter ureolyticus
- GNR
- “Pits” into agar
- pitting of agar, urease positive
Objective 11: List the anaerobic GNRs which will grow on KV (PV) or LKV (KVLB)
Fustobacterium spp. (except F. nucleatum)
Bacteroides spp.
Prevotella spp.
Objective 3: Identify the Gas Liquid Chromatography (GLC) patterns of the following:
Peptostreptococcus anaerobius
Objective 3: Identify the Gas Liquid Chromatography (GLC) patterns of the following:
Fusobacterium spp.
Objective 3: Identify the Gas Liquid Chromatography (GLC) patterns of the following:
Bacteroides fragilis ‘group’
