Amylas Flashcards
Measure the decrease in absorbance of a tubid starch
Turbidimetric
Simple and rapid
Turbidimetric
Disadvantage:
Poor precision in near normal activity of amylase
Inadequate subrate stability
Turbidimetric
Turbidimetric change is not linear to amylase activity, except the first _______ of the reaction
Two minutes
Amylase activity is measured by determining the time required for the amylase to completely hydrolyze all-iodine bound starch
Iodometric
Dark blue colored complex
Iodometric
Indication of the endpoint of iodometric method
Absence of blue starch-iodine color
Gives a blue color with iodine
Amylose
Forms a weak red color
Amylopectin
This is due to the helical configuration formed by amylose with iodine
Formation of blue color
In iodometric method, the blue color is obtained only when the overall chain length of amylose is greater than _________
45 glucose units
Acute pancreatitis
Rise: ?
Peak: ?
Return to normal: ?
R: 5-8h
P: 24h
Rtn: 3-5d
Based on the reduction of light scattering of substrate by amylose activity
Nephelometric
Nephelometric method has the advantage of (?)
Simplicity
Speed
Precision
Zero-order kinetics
Suitable for emergency purposes, results available less than three minutes
Nephelometric
Bench-working time and manipulation is minimal. Does not require blanking
Nephelometric
Nephelometric method standardization requires arbitrary standards such as (?)
Formazine
Major tissue sources of amylase
Pancreas and salivary glands
Minor tissue source of amylase
Small intestine
fallopian tubes
skeletal muscle
Amylase isoenzyme present in normal urine
P-type isoenzyme
S-type isoenzyme is present in (?) of normal serum
2/3
Separation basis of amylase determination
Physical characteristics/difference
Chromatography
Electrophoresis
Iso-electric focusing
Immune inhibition
Immuno precipitation
Selective inhibition
Hydrolysis of starch to produce carbohydrates with reducing properties
Saccharogenic
In saccharogenic method, the activity of amylase if expressed by
Somogyi unit
What is Somogyi unit
Expression on the number of milligrams of glucose released in 30mins at 37°C