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MTAP 1 > Amoeba > Flashcards

Amoeba Flashcards

1
Q

One Nucleus
Peripheral chromatin: Fine, uniform, beaded appearance
Karyosome: Small, Central

A

E. Histolytica/Dispar

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2
Q

Cytoplasm - Fine, granular, ground glass appearance “Clean looking”

A

E. Histolytica/Dispar

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3
Q

Inclusion: Ingested RBC

A

E. Histolytica

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4
Q

Motility: Progressive, Directional (explosive), hyaline fingerlike or blade pseudopods

A

E. Histolytica/Dispar

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5
Q

Small race E. Histolytica

A

E. harmanii

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6
Q

One Nucleus
Peripheral chromatin: Fine, uniform, beaded appearance
Karyosome: Small, Eccentric

A

E. hartmanii

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7
Q

Cytoplasm: Finely Granular

A

E. hartmanii

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8
Q

Motility: Non-progressive

A

E. hartmanii

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9
Q

One nucleus
Peripheral chromatin: Dark Solid Ring
Karyosome: Large, Eccentric

A

E. coli

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10
Q

Cytoplasm: Coarse, vacuolated “dirty looking”

A

E. coli

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11
Q

Motility: Non-progressive(sluggish) Blunt, Granular pseudopod

A

E. coli

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12
Q

One Nucleus
Peripheral Chromatin: closely packed, finely granular
Karyosome: Small, well-defined, central

A

E. gingivalis

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13
Q

Cytoplasm: Finely granular, vacuolated
Inclusions: Epithelial Cells, WBCs

A

E. gingivalis

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14
Q

Motility: Moderately active
Varied pseudopodia

A

E. gingivalis

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15
Q

Smallest intestinal protozoan

A

E. nana

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16
Q

One nucleus
No peripheral chromatin
Karyosome: irregular, large, blot-like, central

A

E. nana

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17
Q

Cytoplasm: Granular, vacuolated

A

E. nana

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18
Q

Motility: Sluggish, non-progressive hyaline, blunt pseudopod

A

E. nana

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19
Q

Basket of flowers

A

I. butschlii

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20
Q

One nucleus
No peripheral chromatin
Karyosome: large, central, surrounded by refractile achromatic granules

A

I. butschlii

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21
Q

Cytoplasm: Coarsely granular, heavily vacuolated

A

I. butschlii

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22
Q

Motility: sluggish, non-progressive, Hyaline, Blunt or fingerlike pseudopod

A

I. butschlii

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23
Q

1-4 Nucleus
Peripheral chromatin: Fine, uniform
Karyosome: Small, Central

A

E. Histolytica/Dispar

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24
Q

Cytoplasm
Chromatoidal bodies: Elongated bars with blunt rounded ends
Cigar/rod/sausage shaped

A

E. Histolytica/Dispar

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25
Q

Shape: Spherical

A

E. Histolytica/Dispar
E. hartmanii
E.coli

26
Q

1-4 Nucleus

A

E. hartmanii

27
Q

Cytoplasm
Chromatoidal bodies: Elongated bars with blunt rounded ends
(bar like or rice-grains)

A

E. hartmanii

28
Q

Way to differentiate E.hartmanii from E.hystolytica

A

Hartmanii has a smaller size trophozoites

29
Q

1-8 Nucleus
Peripheral Chromatin: Coarse Granular, irregular
Karyosome: Large, eccentrics

30
Q

Cytoplasm
Chromatoidal bodies: with pointed ends (Splinter-like broomstick) jagged ends

31
Q

No cyst stages

A

E.gingivalis

32
Q

1-4 nucleus (cross-eyed cyst)
Button hole nucleus
No peripheral chromatin
Karyosome: Large, blot-like, central

33
Q

No Chromatoidal bodies
Granules or small oval masses (comma, coccoid, short curved rods)

34
Q

Shape: Spherical, oval, ellipsoidal

35
Q

1 nucleus
Peripheral chromatin: none
Karyosome: large, eccentric, with refractile achromatic granules (periendosome)

A

I.butschlii

36
Q

No chromatoidal bodies
Glycogen vacuoles
Compact, well-defined (dark brown in iodine prep)

A

I.butschlii

37
Q

Swine amoeba

38
Q

Infective stage of E. histolytica

A

Quadrinucleated cyst

39
Q

Diagnostic stage of E. histolytica in watery stool

A

Trophozoite

40
Q

Diagnostic stage of E. histolytica in formed stool

41
Q

Diagnostic stage of E. histolytica in extraintestinal

A

Trophozoite

42
Q

E.histolytica can cause

A

Amebiasis
Amebic dysentery
Extraintestinal amebiasis (liver, brain, skin, lungs)

43
Q

Incidence of E. histolytica

A

Common in tropics and subtropics
5% incidence in U.S (carrier state)

44
Q

E. histolytica infection often accompanied by

A

Charcot-Leyden crystals

45
Q

Charcot-Leyden crystals indicates?

A

Eosinophilic inflammation

46
Q

Gold standard diagnosis of E. histolytica

A

Concentration technique

47
Q

Culture media for E. histolytica

A

Boeck/Drbohlav’s diphasic
Locke’s egg medium
Shaffer Ryden Frye medium
Balamuth’s medium
Robinson and inoki

48
Q

Lugol’s iodine stains what in E. histolytica?

49
Q

Quensel’s methylene blue stains what in E. histolytica?

A

Trophozoite

50
Q

Other ways to find E. histolytica (diagnosis)

A

Immunofluorescence
PCR

51
Q

Trophozoites are extremely variable in shape and size

A

Acanthamoeba

52
Q

Spine Hyaline extensions

A

Acanthamoeba

53
Q

No flagellate form

A

Acanthamoeba

54
Q

Motility: Sluggish, polydirectional, spine like pseudopods

A

Acanthamoeba

55
Q

10 um double-walled, wrinkled cyst

A

Acanthamoeba

56
Q

Cause GAE (Granulomatous Amebic Encephalitis)

A

Acanthamoeba

57
Q

Causes Keratitis in persons who use daily-wear or extended-wear contact lenses or who have experienced trauma to the cornea

A

Acanthamoeba

58
Q

Chronic granulomatous lesions
Trophozoites and cysts lesions

A

Acanthamoeba

59
Q

Infective stage of Acanthamoeba

A

Trophozoites

60
Q

Diagnostic stage of Acanthamoeba

A

Trophozoites and Cysts

61
Q

Ways to diagnose Acanthamoeba

A

Calcofluor white stain - is a rapid method for the detection of many yeasts and pathogenic fungi such as Microsporidium, Acanthamoeba, Pneumocystis, Naegleria, and Balamuthia species.
Culture:
PYGC media
Bacteria-seeded agar “E.coli”

62
Q

Bonus muna

MTAP 1 (21 decks)

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