Amoeba Flashcards by Potato Patata

One Nucleus
Peripheral chromatin: Fine, uniform, beaded appearance
Karyosome: Small, Central

E. Histolytica/Dispar

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Cytoplasm - Fine, granular, ground glass appearance “Clean looking”

E. Histolytica/Dispar

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Inclusion: Ingested RBC

E. Histolytica

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Motility: Progressive, Directional (explosive), hyaline fingerlike or blade pseudopods

E. Histolytica/Dispar

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Small race E. Histolytica

E. harmanii

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One Nucleus
Peripheral chromatin: Fine, uniform, beaded appearance
Karyosome: Small, Eccentric

E. hartmanii

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Cytoplasm: Finely Granular

E. hartmanii

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Motility: Non-progressive

E. hartmanii

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One nucleus
Peripheral chromatin: Dark Solid Ring
Karyosome: Large, Eccentric

E. coli

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Cytoplasm: Coarse, vacuolated “dirty looking”

E. coli

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Motility: Non-progressive(sluggish) Blunt, Granular pseudopod

E. coli

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One Nucleus
Peripheral Chromatin: closely packed, finely granular
Karyosome: Small, well-defined, central

E. gingivalis

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Cytoplasm: Finely granular, vacuolated
Inclusions: Epithelial Cells, WBCs

E. gingivalis

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Motility: Moderately active
Varied pseudopodia

E. gingivalis

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Smallest intestinal protozoan

E. nana

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One nucleus
No peripheral chromatin
Karyosome: irregular, large, blot-like, central

E. nana

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Cytoplasm: Granular, vacuolated

E. nana

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Motility: Sluggish, non-progressive hyaline, blunt pseudopod

E. nana

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Basket of flowers

I. butschlii

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One nucleus
No peripheral chromatin
Karyosome: large, central, surrounded by refractile achromatic granules

I. butschlii

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Cytoplasm: Coarsely granular, heavily vacuolated

I. butschlii

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Motility: sluggish, non-progressive, Hyaline, Blunt or fingerlike pseudopod

I. butschlii

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1-4 Nucleus
Peripheral chromatin: Fine, uniform
Karyosome: Small, Central

E. Histolytica/Dispar

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Cytoplasm
Chromatoidal bodies: Elongated bars with blunt rounded ends
Cigar/rod/sausage shaped

E. Histolytica/Dispar

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Shape: Spherical

E. Histolytica/Dispar E. hartmanii E.coli

1-4 Nucleus

E. hartmanii

Cytoplasm Chromatoidal bodies: Elongated bars with blunt rounded ends (bar like or rice-grains)

E. hartmanii

Way to differentiate E.hartmanii from E.hystolytica

Hartmanii has a smaller size trophozoites

1-8 Nucleus Peripheral Chromatin: Coarse Granular, irregular Karyosome: Large, eccentrics

E.coli

Cytoplasm Chromatoidal bodies: with pointed ends (Splinter-like broomstick) jagged ends

E.coli

No cyst stages

E.gingivalis

1-4 nucleus (cross-eyed cyst) Button hole nucleus No peripheral chromatin Karyosome: Large, blot-like, central

E.nana

No Chromatoidal bodies Granules or small oval masses (comma, coccoid, short curved rods)

E.nana

Shape: Spherical, oval, ellipsoidal

E.nana

1 nucleus Peripheral chromatin: none Karyosome: large, eccentric, with refractile achromatic granules (periendosome)

I.butschlii

No chromatoidal bodies Glycogen vacuoles Compact, well-defined (dark brown in iodine prep)

I.butschlii

Swine amoeba

E.polecki

Infective stage of E. histolytica

Quadrinucleated cyst

Diagnostic stage of E. histolytica in watery stool

Trophozoite

Diagnostic stage of E. histolytica in formed stool

Cyst

Diagnostic stage of E. histolytica in extraintestinal

Trophozoite

E.histolytica can cause

Amebiasis Amebic dysentery Extraintestinal amebiasis (liver, brain, skin, lungs)

Incidence of E. histolytica

Common in tropics and subtropics 5% incidence in U.S (carrier state)

E. histolytica infection often accompanied by

Charcot-Leyden crystals

Charcot-Leyden crystals indicates?

Eosinophilic inflammation

Gold standard diagnosis of E. histolytica

Concentration technique

Culture media for E. histolytica

Boeck/Drbohlav's diphasic Locke's egg medium Shaffer Ryden Frye medium Balamuth's medium Robinson and inoki

Lugol's iodine stains what in E. histolytica?

Cysts

Quensel's methylene blue stains what in E. histolytica?

Trophozoite

Other ways to find E. histolytica (diagnosis)

Immunofluorescence PCR

Trophozoites are extremely variable in shape and size

Acanthamoeba

Spine Hyaline extensions

Acanthamoeba

No flagellate form

Acanthamoeba

Motility: Sluggish, polydirectional, spine like pseudopods

Acanthamoeba

10 um double-walled, wrinkled cyst

Acanthamoeba

Cause GAE (Granulomatous Amebic Encephalitis)

Acanthamoeba

Causes Keratitis in persons who use daily-wear or extended-wear contact lenses or who have experienced trauma to the cornea

Acanthamoeba

Chronic granulomatous lesions Trophozoites and cysts lesions

Acanthamoeba

Infective stage of Acanthamoeba

Trophozoites

Diagnostic stage of Acanthamoeba

Trophozoites and Cysts

Ways to diagnose Acanthamoeba

Calcofluor white stain - is a rapid method for the detection of many yeasts and pathogenic fungi such as Microsporidium, Acanthamoeba, Pneumocystis, Naegleria, and Balamuthia species. Culture: PYGC media Bacteria-seeded agar "E.coli"

Bonus muna

Bonus