Acetyl CoA and the CAC Flashcards
What is the metabolic fuel for the CAC?
acetyl CoA
acetate+coenzyme A =?
acetyl CoA
What 3 things make up coenzyme A?
- AMP with extra P group attached to ribose
- phosphorylated pantothentic acid (B5)
- mercaptoethyalimine
The reduced thiol (SH) in coASH can react with what to form what type of linkages?
react with carboxyl groups to form thioester linkages
Acetyl Coa can be formed from what 3 things? Acetyl Coa in turn can be used for what 4 processes?
carbs, lipids, amino acids
- CAC
- Fatty acid biosynthesis: TAG, Phospholipids
- Ketone Body formation
- Cholesterol biosynthesis: steroid hormones, bile acids, vit D
Biochemical reaction for oxidative decarboxylation of pyruvate?
Pyruvate + NAD+ + COASH –> PDH –> acetyl CoA + NADH + CO2 + H+
What 3 enzymes make up the PDH complex?
pyruvate decarboxylate: (Ena) dihydrolipoyl transacetylase (Enb) dihydrolipoyl DH (EnC)
What is vitamin B1?
thiamine
Where is the PDH complex located?
mitochondrial matrix
Where is the pyruvate from glycolysis generated?
in the cytosol
Where is the protein translocase for pyruvate located?
in the inner mit membrane
What are the 5 coenzymes for the PDH complex?
2 soluble: Coa and NAD+
3 tighly bond: TPP, lipoamide, FAD
What 4 B vits are required for PDH complex?
- Pantothenic acid (B5) - CoAsh
- Thiamin (B1) - TPP
- Niacin (B3)- NAD+
- Riboflavin (B2) (FAD)
What is riboflavin required for?
it is B2.. and FAD needs it
What is B2?
riboflavin
What B vitamin does NAD+ require?
niacin
Draw out the steps of the CAC!!! anddddd GO :)
:)
What 3 steps produce NADH?
- isocitrate + NAD+ –> isocit DH –> aKG + NADH+CO2+H
- aKG + CoASH+ NAD+ –> aKG DH –> succinyl-COA + CO2 + NADH+ H+
- malate + NAD+ –> mal DH –> OAA + NADH + H