AAV

Question 1

AAV structure & classification

Answer 1

Family: Parvoviridae
Genus: Dependoparvovirus
Species: Adeno-associated dependoparvovirus A &
Adeno-associated dependoparvovirus B

ssDNA NON enveloped

Baltimore: II

Question 2

AAV hepatitis burden

Answer 2

In April 2022, several hospitals in Scotland reported that children were presenting to medical practitioners with acute severe hepatitis of unknown aetiology1 (Fig. 1a). Elsewhere in the United Kingdom, 270 similar presentations were subsequently reported, for which 15 children required liver transplantation3. WHO has now registered 1,010 probable cases that fulfil their definition of this illness in 35 countries.

Case control study performed in Scotland - Near-full genomes of AAV2 were obtained from a large proportion patients with hepatitis by NGS. Several mutations within the VP1–VP3 genes were noted to be over-represented in the sequences derived from patients with hepatitis when compared with reference sequences.

FISH for AAV2 in liver biopsy.