A2- Chromatography Flashcards

1
Q

What is chromatography?

A

chemical separation technique that allows separation of mixtures

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2
Q

What is the basic principle of all chromatography?

A

components of a mixture dissolved in a fluid will flow through another material/stationary phase

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3
Q

What does separation depend on?

A
  • interaction with stationary phase
  • solubility in mobile phase
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4
Q

What is the stationary and mobile phase for TLC?

A

Stationary: Silica coated plastic plate
Mobile: liquid solvent

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5
Q

What is the stationary and mobile phase for gas chromatography?

A

Stationary: microscopic liquid film on solid support
Mobile: INERT carrier gas

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6
Q

What is the stationary and mobile phase for column chromatography?

A

Stationary: solid silica
Mobile: liquid solvent

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7
Q

What do the solute molecules do?

A

adsorb to the stationary phase

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8
Q

What does the distance travelled depend on?

A

the interactions between the component in the mobile phase and the stationary phase

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9
Q

What can be used to locate spots?

A
  • UV
  • Ninhydrin
  • iodine vapour
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10
Q

Why might 2 dimensional TLC be used?

A

as some amino acids have similar Rf values in different solvents

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11
Q

How would increased solubility due to a more polar solvent impact Rf value?

A

increase Rf value
as component is more soluble in mobile phase, so travels further up the stationary phase

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