9 - Order of Draw and tubes

Question 1

What is the order of draw for a venipuncture?

Answer 1

Yellow
Lt Blue
Red or Orange
Green
Lavender
White 
Gray
Y,B,R,Gn,L,W,Gy

1. Blood gases
2. Sodium citrate - blood thinner test
3. Hematology - no additive
4. Heparin - Green
5. EDTA - Lavender, pink, royal, tan
6. Sodium floride - gray

Question 2

What is the additive in a yellow tube?

Answer 2

None

Used for an intermediate for blood gases, we do not do this, we use the butterfly to the aerobic and anaerobic bottles.

Question 3

What is the additive in a Red tube & what is the use?

Answer 3

NO Additive
Serum tube
Therapeutic Drug Monitoring TDM
Infectious disease monitoring

Question 4

What is the additive in a Light Blue tube and the use?

Answer 4

Sodium Citrate
Use is PT/APT
Blood coagulation testing

Question 5

What is the additive in the green tube and the use?

Answer 5

Lithium Heparin
Glucose
BUN - Blood Urea Nitrogen
Ionized Calcium
Creatine
Electrolyte studies

Question 6

What is the additive lavender tube and what is tested?

Answer 6

EDTA

Used for Hemotalogy tests: CBC, Differential, Hemocrit, hemoglobin, RBC, WBC, MCHC, platelet

Question 7

What are some concerns with an under-filled lavender tube?

Answer 7

False low cell counts
False low hemocrits
Staining alterations on blood smears
Erroneous morphological change in RBC

Question 8

What is the gray tube used for?

Answer 8

It has a gylcolic inhibitor, so it is used for delayed glucose testing (max 24 hours).

Question 9

What is a royal blue tube used for?

Answer 9

Toxology

Question 10

What is a tan tube used for?

Answer 10

Lead testing

Question 11

Order of collection capillary puncture?

Answer 11

1. Blood gases (ABG)
2. EDTA (Lavender, Royal, pink, tan)
3. Other additives (green)
4. Non-additives (red)

Question 12

What is liquid vs. clotted?

Serum is __________
Plasma is __________

Answer 12

Serum is clotted

Plasma is liquid