8A Mutations and Gene Expression Flashcards
How can hypomethylation lead to cancer?
It switches on the oncogene
Which type of tumourc an metastatise?
Malignant
Give an example of where you may find a multipotent stem cell
Bone marrow
What is the science of collecting and analysing complex biological data called?
Bioinformatics
What type of tumour is not surrounded by a capsule
Malignant
What do we call molecules that move into the nucleus and switch genes on?
Transcriptional factors
What is the purpose of whole-genome shotgun sequencing?
Cut the genome into lots of pieces to then later reassemble
If the code has a base added or taken away, this will produce a…
Frame shift
If the gene is inaccessible, associated histones have…
Decreased acetylation
If the gene is accessible, the cytosine in DNA have…
Decreased methylation
What type of gene mutation separates a DNA sequence which then rejoins on a different chromosome?
Translocation
Multipotent stem cells can differentiate into _______ type of cell
Limited
What process does siRNA inhibit?
Translation
What type of gene mutation separates a DNA sequence which then rejoins in the same place but in the reverse order?
Inversion
What type of gene mutation removes a nucleotide?
Deletion
If the gene is accessible, associated histones have…
Increased acetylation
Give an example of where you may find a unipotent stem cell
Adult tissue
What is the proteome?
All the proteins produced by the genome
If the gene is inaccessible, the cytosine in DNA has…
Increased methylation
What type of tumour produces adhesion molecules?
Benign
What type of gene mutation replaces one nucleotide with another?
Substitution
What effect does oestrogen have on transcription?
Activates it
What type of stem cell is an iPS cell produced from?
Unipotent
What type of chromatin is tightly packed?
Heterochromatin
Hypermethylation can lead to cancer because…
It switches off the tumour supressor gene
The epigenome forms a layer around the DNA and histones made up of what?
Chemical tags
What type of chromatin is loosely packed?
Eurochromatin
What is a SNP?
A single base variation in the genome
Give an example of where you may find a pluripotent stem cell
Embryo
What type of tumour has systemic effects?
Malignant
Give an example of where you may find a totipotent stem cell
Zygote/early embryo
What type of gene mutation repeats a base?
Duplication
Pluripotent stem cells can differentiate into _______ type of cell
Almost any
Unipotent stem cells can differentiate into _______ type of cell
Single
What type of tumour rarely reoccurs after treatmen?
Benign
What type of gene mutation inserts an extra base in the sequence?
Addition
What factors can the epigenome be influenced by?
- Stress
- Diet
- Toxis
A mutated proto-oncogene causes the cell to…
Rapidly divide
A mutated tumour suppressor gene causes the cell to…
Rapidly divide
Does changing the epigenome change the sequence of bases in DNA?
No
What chemical acts normally as a transcriptional factor, but increased amounts can lead to tumour formation?
Oestrogen
Totipotent stem cells can differentiate into _______ type of cell
Any
What are mutations?
Changed to the base sequence of DNA
What can cause mutations?
Errors during DNA replication
What can increase the rate of mutations?
Mutagenic agents
What are the types of mutations that can occur?
- Substitution
- Deletion
- Addition
- Duplication
- Inversion
- Translocation
What is a substitution mutation?
One or more bases are swapped for another
e.g. ATGCCT becomes ATTCCT
What is a deletion mutation?
One or more bases are removed
e.g. ATGCCT becomes ATCCT