8/23/13

Question 1

Linear epitope

Answer 1

epitope consists of a linear string of 8-20 amino acids

Question 2

Discontinous or conformational epitope

Answer 2

amino acids in the epitope may be from distant parts of the linear peptide

Question 3

T-cells (can/cannot) recognize discontinous epitope

Answer 3

cannot

Question 4

Papain digests antibody to produce a Fab fragment that has a (monovalent/bivalent) binding to antigens

Answer 4

monovalent

Question 5

Pepsin digests antibody to produce a Fab fragment that has a (monovalent/bivalent) binding to antigens

Answer 5

bivalent

Question 6

N-terminus/C-terminus is where the variable region of the antibody is located

Answer 6

N-terminus

Question 7

List of Ig heavy chain genes

Answer 7

alpha, delta, gamma, epsilon, mu

Question 8

List of Ig light chain genes

Answer 8

kappa, lambda

Question 9

Which antibody types are the only ones to act as B-cell receptors

Answer 9

IgD, IgM

Question 10

How many CDRs are there for each heavy and light chain in the variable region of the antibody?

Answer 10

3

Question 11

Which interleukin is required for lymphocyte maturation?

Answer 11

7

Question 12

Light chains and alpha chains have only these cassettes

Answer 12

V-J

Question 13

D-cassettes occur only in these types of lymphocyte receptor segments

Answer 13

heavy chain genes, beta genes of the TCR

Question 14

Which segments are joined during somatic recombination are random is called this

Answer 14

combinatorial diversity

Question 15

Junctional diversity

Answer 15

Ligases add random nucleotides to the join sites

Question 16

This protein activates the somatic recombination complex in immature lymphocytes

Answer 16

RAG

Question 17

IgD or IgM can be on the BCR because they come from the same mRNA but are

Answer 17

alternatively spliced

Question 18

Describe how antibody chains are selected during B cell maturation (allelic maturation)

Answer 18

One heavy chain undergoes VDJ recombination–> if unsuccessful the other allele attempts–> if successful, the protein made prevents the other chromosome from recombining and indues kappa light chain locus to start VDJ recombination–> if both kappa alleles fail then lambda has a shot–> if all fails the cell dies

Question 19

What type of B-cell is in the fetus and where is it located?

Answer 19

B-1, liver

Question 20

Describe how T-cell receptors are selected

Answer 20

Pro-T-cells recombine one beta chain gene–> if productive, prevents the other chromosome from rearranging and induces alpha chain recombination–> if productive CD4 and CD8 are induced–> if weak reaction with MHC II + peptide, then CD4 is selected–> if weak reaction with MHC I +peptide, then CD8 is selected

Question 21

Where will you only see double positive T-cells

Answer 21

thymus