6 - RNA tertiary structure Flashcards
(27 cards)
In RNA, the primary structure is
the sequence of nucleotides
In RNA, the secondary structure is
where WC base pairs form and where they don’t
In RNA, the tertiary structure is
how the secondary structural elements pack together in 3-dimensions
In RNA, P, J, and L stand for
paired region, junction, loop
Compensatory mutagenesis makes
mutations to disrupt a potential helix
For most RNAs, the tertiary structure is ______ dependent
metal ion
Why is RNA better than DNA at forming complex tertiary interactions?
The ability of the 2’-hydroxyl groups to H-bond
RNA structures are _______ cooperative than protein structures
less
In RNA, tertiary structure interactions result from
base stacking and hydrogen bonding
RNA is very prone to
misfolding
RNA helix formation tends to be ________ driven
enthalpically
A reverse G-U wobble dramatically
changes the nature of the minor groove
In RNA, non-WC base pairs facilitate
tertiary structure formation
A WC ______ base pair cannot substitute for a G-U wobble
G-C
Minor groove - minor groove interactions in RNA are
very common
Tetraloops can dock in
the minor groove of RNA helices
The major groove is ______ for packing
inaccessible
In a tetraloop, the G and N nucleotides are ____ bases away in the helical stack
2
The G and A in a GNRA tetraloop form a
sheared pair
In a G-A sheared pair, the A
slips below and across the G
The tetraloop receptor makes specific contacts to _______ in the GAAA tetraloop
the first A
The G-A sheared base pair positions an adenosine in
the minor groove of the RNA helix
Adenosines have a shape complementary to the minor groove of a
G-C base pair
Ribose zippers
H-bonds involving the 2’-hydroxyls on consecutive base pairs
