5 - Structure and function of nucleic acids

Question 1

Do purines or pyrimidines base stack better?

Answer 1

purines

Question 2

Proteins that bind DNA usually do so in the

Answer 2

major groove

Question 3

Conversion from B-DNA to A-DNA occurs under

Answer 3

low-hydration conditions

Question 4

Why can’t RNA adopt a B-form geometry?

Answer 4

the 2’-hydoxyl would hit the following sugar

Question 5

Double stranded RNA adopts _______ helix

Answer 5

A-form

Question 6

A-form helices have a deep, inaccessible

Answer 6

major groove

Question 7

RNA-protein interactions usually occur in the

Answer 7

minor groove

Question 8

Stem-loops are stabilized by

Answer 8

base-pairing, stacking, and number of mismatches

Question 9

Stem-loops cannot be less than _____ bases

Answer 9

3

Question 10

In a G-U wobble, G is shifted towards the

Answer 10

minor groove

Question 11

In a G-U wobble, the U is shifted towards the

Answer 11

major groove

Question 12

An isosteric A-C wobble can for if

Answer 12

the A is protonated

Question 13

Tetraloops consist of ______ nucleotides

Answer 13

4

Question 14

Tetraloop possible sequences

Answer 14

GNRA
UNCG
CUUG

Question 15

In a tetraloop, the 1st and 4th unpaired nucleotides form

Answer 15

non-WC base pairs

Question 16

Loops and single stranded regions of RNA tend to contribute to

Answer 16

tertiary structure formation

Question 17

Internal loops tend to make

Answer 17

WC base pairs

Question 18

3’-overhangs are

Answer 18

stabilizing

Question 19

Loops are often

Answer 19

destabilizing