2 - Membrane proteins

Question 1

3 classes of membrane lipids

Answer 1

phospholipids, cholesterol, and glycolipids

Question 2

The _________ nature of lipids is essential to membrane structure

Answer 2

amphiphilic

Question 3

Lipid hydrophilic domains are called

Answer 3

headgroups

Question 4

The type of lipid aggregate that spontaneously forms is a result of the

Answer 4

overall hydrophobicity and structure of the component lipid building block

Question 5

Clustering of lipids to form structures is due to

Answer 5

the hydrophobic effect

Question 6

Cone-shaped lipid molecules form

Answer 6

micelles

Question 7

Cylinder-shaped lipid molecules form

Answer 7

bilayers

Question 8

Lipid molecules spontaneously form one or the other structure in water, depending on

Answer 8

their shape

Question 9

Detergents are

Answer 9

surfactants or surface active molecules that reduce surface tension

Question 10

T/F Detergents are amphipathic molecules

Answer 10

True

Question 11

Detergents self associate in a concentration dependent fashion to form

Answer 11

multimolecular structures called micelles

Question 12

In detergents, the head is ______ while the tail is ______

Answer 12

polar; hydrophobic

Question 13

CMC stands for

Answer 13

critical micelle concentration

Question 14

CMC is the

Answer 14

lowest concentration of detergent above which monomers form micelles

Question 15

CMC _______ with increase in alkyl chain length

Answer 15

decreases

Question 16

For ionic detergents, CMC decreases with

Answer 16

increasing counterion concentration

Question 17

CMC __________ with introduction of double bonds

Answer 17

increases

Question 18

The cost of partitioning non-hydrogen-bonded peptide bonds into completely apolar phases is

Answer 18

very high

Question 19

The cost of partitioning is dramatically reduced when peptide bonds participate in

Answer 19

hydrogen bonds

Question 20

Reducing the cost of partitioning is a major driving force for the formation of _________ in membranes

Answer 20

secondary structure

Question 21

Membrane proteins are _____ to crystallize

Answer 21

difficult

Question 22

Must use _______ to solubilize and purify membrane proteins

Answer 22

detergents

Question 23

Addition of ______ molecules can aid crystallization

Answer 23

amphipathic

Question 24

Attach ______ to facilitate solubilization

Answer 24

antibodies

Question 25

Ways to study membrane proteins in vitro

Answer 25

Detergents
Proteoliposomes
Nanodiscs

Question 26

Membrane protein structure formation is dominated by

Answer 26

hydrogen-bonding interactions

Question 27

The low dielectric constant found in the nonpolar domain of the lipid bilayer coupled with the lack of water molecules greatly favors

Answer 27

the formation of secondary structures