4 - Nucleic acids introduction Flashcards
1
Q
Number of different mammalian mRNAs
A
12,000
2
Q
Number of mammalian housekeeping mRNAs
A
10,000
3
Q
Human genome contains how many protein coding genes
A
26,000
4
Q
Genome exons %
A
1.1
5
Q
Genome introns %
A
24
6
Q
Genome intergenic DNA %
A
75
7
Q
3 components of nucleotides
A
- 5 carbon sugar
- one or more phosphate group
- base
8
Q
RNA nucleosides
A
- adenosine
- guanosine
- cytidine
- uridine
9
Q
DNA nucleosides
A
- deoxyadenosine
- deoxyguanosine
- deoxycytidine
- thymidine
10
Q
Nucleic acid sequences are written from
A
5’ to 3’
11
Q
In B-DNA, bases are ______ apart
A
3.4 A
12
Q
In B-DNA, there are ______ bases per turn
A
10
13
Q
Melting curve measures
A
denaturation
14
Q
ssDNA absorbs ______% more light at 260 nm than dsDNA (due to unstacking)
A
12-40
15
Q
Melting point, Tm, is the temperature at which
A
half the DNA is single stranded
