5C: Separations and Purifications

Question 1

Function of ebulliator

Answer 1

introduce small air bubbles to break surface tension of heated liquid and prevent superheating

Question 2

good example of organic solvent

Answer 2

ether (non-reactive)

Question 3

How does a protein size get effected when run in:
native
non-reducing SDS-PAGE
reducing SDS-PAGE

Answer 3

• native: original structure
• non-reducing: smallest monomers
• reducing: smallest monomers with disulfide bonds broken

Question 4

NMR spectroscopy: further left-shielded (downfield) the peak, the more electron drawing/withdrawing?

Answer 4

electron withdrawing