5. Plasma Membrane Flashcards
Functions of membrane (role of membrane in cells = 18ii)
Compartmentalisation
• Site for attachment of enzymes
• Provide selective permeability
• Create concentration gradients
Site of chemical reactions
Why can glucose not pass through a cell membrane through simple diffusion
Glucose molecules too large
Phospholipids act as a barrier
Function of cholesterol in plasma membrane
Regulates fluidity = sits between tails
Increases strength and stability
Role of membrane in RER (19)
- compartmentalisation
- separating proteins from cell cytoplasm
- hold ribosomes in place
Compartmentalisation
creating organelles within a cell e.g. Mitochondria
What organelles have membranes
Lysosomes
Golgi body
ER
Mitochondria
Chloroplast
Vacuole
Nucleus
Organelles without membrane
Centrioles
Ribosome
Model of membrane name
Fluid mosaic model
Why is the model called fluid
o The phospholipids and proteins can move around via diffusion
o The phospholipids mainly move sideways, within their own layers
Main components in the fluid mosaic model
o Phospholipids o Cholesterol
o Glycoproteins and glycolipids
o Transport proteins - carrier + Channel proteins
What’s the cell surface membrane also known as
Phospholipid bilayer
How are phospholipids arranged in the cell surface membrane
tails form a hydrophobic core comprising the innermost part of both the outer and inner layer of the membrane
Heads face outwards - hydrophilic sides
What does the cell surface membrane act as a barrier to
most water-soluble substances (due to hydrophobic tails)
What does having a membrane that acts as a barrier most water-soluble substances ensure
ensures water-soluble molecules such as sugars, amino acids and proteins cannot leak out of the cell and unwanted water-soluble molecules cannot get in
Can water go through the membrane
Yes kind of
Why can water go through the membrane
Water - very small molecule - can still slowly squeeze its way through, despite being hydrophobic core
What is a micelle
Phospholipids arranged in a spherical form
Why do micelles form
not enough phospholipids to form a full bilayer
Two types of phospholipids
Saturated and unsaturated
What is the difference between saturated and unsaturated phospholipids
saturated fatty acids - straight tails
• unsaturated fatty acids = double bond = - tails with kinks/bends in them
What increases the fluidity of the membrane
Increasing the number of unsaturated fatty acids / phospholipids
Why does increasing the number of unsaturated fatty acids increase the fluidity of the membrane
more unsaturated fatty acids = more distance between the tails and thus
fewer intermolecular interactions = more fluidity
How does cholesterol stop the membrane from becoming to fluid at high temps
Cholesterol molecules bind to the hydrophobic tails of phospholipids, stabilising them and causing phospholipids to pack more closely together= decreasing fluidity
Extrinsic proteins
proteins which are found on the surface of the plasma membrane.
General function of extrinsic proteins
usually function as enzymes and catalyse chemical reactions inside the cell.
what are Intrinsic proteins
proteins which span both bilayers of the plasma membrane.
General function of intrinsic proteins
act as channels or carrier proteins to transport water-soluble molecules.
What are glycoproteins
Proteins Embedded in the cell-surface membrane with attached carbohydrate (sugar) chains of varying lengths and shapes.
Function of glycoproteins
Cell Signalling
Recognition sites
Example of cell signalling
Receptors for peptide hormones, inc. insulin and glucagon, which affect the uptake/storage of glucose by cells.
What type of proteins are glycoproteins
Intrinsic proteins
What are glycolipids
lipids with attached carbohydrate (sugar) chains
Function of glycolipids
Recognition sites / antigens
can be recognised by cells of immune system as self (of the organism) or non-self (belongs to another organism).
What are extrinsic proteins also known as
Peripheral protein
Function of transport proteins
create hydrophilic channels to allow ions and polar molecules to travel through the membrane.
Types of transport proteins
Channel protein
Carrier protein
Function of channel proteins
Transport polar substances down a concentration gradient
allow the cell to control which substances enter or leave
Function of carrier proteins
Transport molecules against the concentration gradient
allow the cell to control which substances enter or leave
Adaption of carrier proteins
change shape to transport a substance across the membrane
Can any molecule go down any channel / carrier protein
No = • Each transport protein is specific to a particular ion or molecule
What two factors effect membrane permeability
Temp + solvent concentration
Initial effect of increase temp on membrane permeability
• Phospholipids in a cell membrane are constantly moving.
• When temp. increased, the phospholipids have more kinetic energy = move more.
• This makes a membrane more fluid, and it will start to lose structure =
REVERSIBLE
Later effect of increased temp on membrane permeability
Temp. continues to increase = cell will break down completely.
• Loss of structure increases permeability of membrane, making it easier for particles to cross it.
• Carrier and channel proteins in membrane will be denatured at high temps.
These proteins -> involved in transport across membrane = so as they denature
= membrane permeability is affected. = IRREVERSIBILE
What also takes place at increased temps across a membrane
• Any diffusion taking place through the cell membrane will also occur at a higher speed (due to increased kinetic energy)
How does solvent concentration effect membrane permeability
Organic solvents can increase cell membrane permeability as they dissolve the lipids in the membrane, causing the membrane to lose its structure
Define diffusion
the net passive movement of molecules from an area of high concentration to an area of low concentration
What happens after a long period of diffusion
Reaches equilibrium
How does temp effect rate of diffusion
Molecules and ions have more kinetic energy at higher temperatures
• They move faster, resulting in a higher rate of diffusion
How’s does the concentration gradient effect rate of diffusion
o This is the difference in the concentration of the
substance on the two sides of the surface o If there are more molecules on one side of a membrane than on the other, at any one moment more molecules will randomly move across the membrane from that side than from the other
• A greater difference in concentration means a greater difference in the number of molecules passing in the two directions and therefore a faster rate of diffusion
How does SA effect the rate of diffusion
The greater the surface area across which diffusion is taking place, the greater the number of molecules or ions that can cross it at any one moment and therefore the faster diffusion occurs
How can you increase the SA of an organelle / organism
Or decreasing it’s size
• The surface area of cell membranes can be increased by folding (eg. microvilli in the intestine or cristae in mitochondria)
How does the properties of the molecules or ions dissolving effect the rate of diffusion
• Large molecules diffuse more slowly than smaller ones as they require more energy to move
• Uncharged and non-polar molecules diffuse directly across the phospholipid bilayer
• Non-polar molecules diffuse more quickly than polar ones as they are soluble in the non -polar phospholipid bilayer
how do decrease the fluidity of a membrane
An increased proportion of saturated fatty acid chains as the chains pack together tightly and therefore there is a high number of intermolecular forces between the chains
• A lower temperature as the molecules have less energy and therefore are not moving as freely which causes the structure to be more closely packed
how to increase the fluidity of a membrane
• An increased proportion of unsaturated fatty acid chains as these chains are bent, which means the chains are less tightly packed together and there are less intermolecular forces
• At higher temperatures, the molecules have more energy and therefore move more freely, which increasing membrane fluidity
What can no diffuse through phospholipid bilayer
o Large polar molecules such as glucose and amino acids o lons such as sodium ions (Nat) and chloride ions (CI-)