4103FSBMOL - Practical 2 - UV-Vis Analysis. Flashcards
UV-Visible Analysis of a Suspected Date-Rape Sample suspected to contain Ketamine.
What suspected drug were we trying to identify in the sample in this practical?
Ketamine.
How quickly can date-rape drugs act?
Within 20-30 minutes.
What effects can date-rape drugs cause?
- Loss of Inhibitions.
- Muscle Relaxant.
- Sedative.
- Loss of Will to resist.
- Anterograde Amnesia (inability to form memories).
How long do effects last when spiked with Ketamine?
30-45 minutes.
What is the name of the relaxation state for low Ketamine Concentrations?
“K-Land”.
What effects can High Ketamine Concentrations cause?
Dream like states, visual hallucinations, sensations of floating out your own body, confusion, delirium and anterograde amnesia.
What sensation does “K-hole” form?
Near Death Experience.
What is classed as a Low Concentration for Ketamine?
10-75mg/500ml.
What is classed as a High Concentration for Ketamine?
100-250mg/500ml.
What is classed as a “K-hole” Concentration for Ketamine?
500mg/500ml.
How can UV-Visible Spectroscopy be used to analyse colourless solutions of Ketamine?
When the colourless camples absorb UV light, electrons are excited from ground to excited state to make absorption happen in the UV region.
What Law is UV-Visible Spectroscopy goverened by?
Beer-Lambert Law.
What does the Beer-Lambert Law state?
Absorbance is proportionate to Concentration.
What is the Formula for the Beer-Lambert Law?
What does each symbol/ letter stand for?
A = εcl
- A = Absorbance.
- ε = Molar Extinction Coefficient.
- c = Concentration.
- l = path length.
What is the name of the piece of equipment used in this practical?
UV-Visible Spectrophotometer.
What were the standard solutions prepared in by the technicians?
5% ethanol solution.
How much did we have to dilute the Crime Scene Sample down by?
1 in 25 diluted sample using 5% ethanol.
What scanning range do we need to use on the UV-Visible Spectrophotometer?
200-350nm.
What chemical do we need to use as a blank for Practical 2?
Ethanol solution.
What modes on the UV-Visible Spectrophotometer do we need to select?
- Wavescan.
- Single Wavelength Mode.
- Absorption.
- No prompt.
What do each of the buttons do on a Spectrophotometer?
- Blue - Blank Mode.
- Green - Scan Sample.
- Red - Back to Main Menu.
- 2 - Print Result.
What concentration was the stock solution of Ketamine used?
100µg/ml in ethanol.
What wavelength do we have to set the Spectrophotometer to measure the Absorbances of the Blank and Standards?
220nm.
What do we have to plot on the y-axis of the graph?
Absorbance (no units - wavelength 220nm).
