4.1 Lab safety Flashcards
Potential that a chosen action will lead to an undesirable out come
Probability of harm form occurring given a specific situation
Risk
Has the potential for causing harm
Hazard
Risk = Hazard + ______
Situation / action
Types of hazards
Safety / physical
Chemical
Radiological
Biological
Ergonomic
Socio-Cultural
Posture / proper height of stuffs like chair etc.
Ergonomic
Environment/Animal test lab
Stigma for animal testing
Beliefs about animal testing
(Cultural aspect on what you believe in Like cloning) - Does it make you uncomfortable doing this that may hinder your mentality or your reputation?
Bullying
Socio-cultural
Potential source of harm cause by biological materials
Biological hazard
Any material composed of, containing or that may contain biological agents
Biological material
Any microbial entity, cellular or non-cellular that may able to produce infection
Biological agents
Are infections, either symptomatic or asymptomatic, that are acquired through lab or lab-related activities, as a result of working with infectious agent
Laboratory acquired infections
LAI how many percent due to equipment failure
20%
LAI how many percent due human factors lacking
- PPE
- Policies
80%
What are the top 4 accidents resulting to infections?
- Spills and splashes
- Needle and syringe pricks
- Sharp objects, broken glass
- animal scratch or bite
Containment to prevent UNINTENTIONAL accidental release of pathogens
Protects people from bad bugs
Lab biosafety
Prevent loss, theft or INTENTIONAL release of pathogens and toxins
Protects bad bugs from bad people
Lab biosecurity
What are the 3 purpose of biosafety?
Protect
- users
- Those outside the Lab
- Environment
Constituting the national Committee on Biosafety on the Philippines and for other purpose
EO no, 430 s 1990
Constituting the various Biotechnology institutes within the University of the Philippines System as the Network of National Institutes of Biotechnology
Proclamation no. 526, s 1995
Prescribing guidelines and establishing a Regulatory framework for Prospecting of biological and Genetic Resources, Their By-products and Derivatives, for Scientific and Commercial Purposes; and for Other Purposes
EO no 247. s 1995
Establishing the National Biosafety Framework, Prescribing Guidelines for Implementation, Strengthening the National Committee on Biosafety of the Philippines, and for Other Purposes
EO no. 514 s, 2006
We Shall promote the Safe and responsible use of modern biotechnology and its products as one of several means to achieve and sustain food security, equitable access to health services, sustainable and safe environment and industry development
Philippine National Policy Statement on Modern Biotechnology (2001)
What are the 4 Biosafety principles?
- Equipment safety
- Levels of Protection
- Practice and procedures
- Facility Design and Construction
Standard microbiological practices
Lab techniques
Practice and procedures
Containment barrier
Minimize exposure to hazard
Engineering controls
PPE
Equipment safety
Secondary barrier/engineering
controls
• Contributes to worker protection
• Protects outside the laboratory
Facility Design and Construction
Clean benches
Air flow
Filters (HEPA)
Biosafety cabinet (additional airflow - Keeps the bacteria inside)
Laminar flowhood
Meaning of HEPA
High efficiency particulate air filters
Prevents fume from chemicals
Fumehood (Right pic)
Whats the difference between between Biosafety cabinet and Laminar flowhood
Difference biosafety features
High pressure inside the room than outside (Keeps the room clean - Clean room)
Positive pressure
Low pressure in side than outside (Keeps the particulates inside - Dirty room)
Negative pressure
Basic teaching research
GMT
Open work
Biosafety level 1
Primary health services, diagnostic services, research
GMT, PPE, Biohazard sign
BSC for potential aerosols
Biosafety level 2
Special diagnostic services
Airborne bacteria
Controlled access, Directional airflow
Biosafety level 3 (Containment)
Dangerous pathogens (Ebola, Small pox, Etc,)
Class III BSC
Biosafety level 4
Not A recognized containment level in biosafety guidance documents
Conducted in BSL 2 lab with Biosafety practices and procedures typically found at BSL 3
BSL 2+
Assignment of microorganisms into groups based on: Pathogenecity, mode of transmission, preventive measures, treatment
Risk groups
A microorganims that is unlikely to cause harm or disease
No or low individual and community risk
Risk group 1
A pathogen than can cause human or animal disease but no serious to lab workers
Moderate individual risk, Low community risk
Risk Group 2
Serious human or animal disease on one infected human (Blood borne pathogens)
high individual risks, Low community risk
Risk group 3
Serious disease that can spread to the community, treatment may not be available
High on both individual and community risk
Risk group 4
