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Bacte Lab (Prelims) > 4.1 Lab safety > Flashcards

4.1 Lab safety Flashcards

1
Q

Potential that a chosen action will lead to an undesirable out come

Probability of harm form occurring given a specific situation

A

Risk

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2
Q

Has the potential for causing harm

A

Hazard

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3
Q

Risk = Hazard + ______

A

Situation / action

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4
Q

Types of hazards

A

Safety / physical
Chemical
Radiological
Biological
Ergonomic
Socio-Cultural

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5
Q

Posture / proper height of stuffs like chair etc.

A

Ergonomic

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6
Q

Environment/Animal test lab

Stigma for animal testing

Beliefs about animal testing

(Cultural aspect on what you believe in Like cloning) - Does it make you uncomfortable doing this that may hinder your mentality or your reputation?

Bullying

A

Socio-cultural

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7
Q

Potential source of harm cause by biological materials

A

Biological hazard

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8
Q

Any material composed of, containing or that may contain biological agents

A

Biological material

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9
Q

Any microbial entity, cellular or non-cellular that may able to produce infection

A

Biological agents

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10
Q

Are infections, either symptomatic or asymptomatic, that are acquired through lab or lab-related activities, as a result of working with infectious agent

A

Laboratory acquired infections

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11
Q

LAI how many percent due to equipment failure

A

20%

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12
Q

LAI how many percent due human factors lacking
- PPE
- Policies

A

80%

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13
Q

What are the top 4 accidents resulting to infections?

A
  1. Spills and splashes
  2. Needle and syringe pricks
  3. Sharp objects, broken glass
  4. animal scratch or bite
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14
Q

Containment to prevent UNINTENTIONAL accidental release of pathogens

Protects people from bad bugs

A

Lab biosafety

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15
Q

Prevent loss, theft or INTENTIONAL release of pathogens and toxins

Protects bad bugs from bad people

A

Lab biosecurity

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16
Q

What are the 3 purpose of biosafety?

A

Protect
- users
- Those outside the Lab
- Environment

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17
Q

Constituting the national Committee on Biosafety on the Philippines and for other purpose

A

EO no, 430 s 1990

18
Q

Constituting the various Biotechnology institutes within the University of the Philippines System as the Network of National Institutes of Biotechnology

A

Proclamation no. 526, s 1995

19
Q

Prescribing guidelines and establishing a Regulatory framework for Prospecting of biological and Genetic Resources, Their By-products and Derivatives, for Scientific and Commercial Purposes; and for Other Purposes

A

EO no 247. s 1995

20
Q

Establishing the National Biosafety Framework, Prescribing Guidelines for Implementation, Strengthening the National Committee on Biosafety of the Philippines, and for Other Purposes

A

EO no. 514 s, 2006

21
Q

We Shall promote the Safe and responsible use of modern biotechnology and its products as one of several means to achieve and sustain food security, equitable access to health services, sustainable and safe environment and industry development

A

Philippine National Policy Statement on Modern Biotechnology (2001)

22
Q

What are the 4 Biosafety principles?

A
  1. Equipment safety
  2. Levels of Protection
  3. Practice and procedures
  4. Facility Design and Construction
23
Q

Standard microbiological practices

Lab techniques

A

Practice and procedures

24
Q

Containment barrier
Minimize exposure to hazard
Engineering controls
PPE

A

Equipment safety

25
Q

Secondary barrier/engineering
controls
• Contributes to worker protection
• Protects outside the laboratory

A

Facility Design and Construction

26
Q

Clean benches
Air flow
Filters (HEPA)

A

Biosafety cabinet (additional airflow - Keeps the bacteria inside)
Laminar flowhood

27
Q

Meaning of HEPA

A

High efficiency particulate air filters

28
Q

Prevents fume from chemicals

A

Fumehood (Right pic)

29
Q

Whats the difference between between Biosafety cabinet and Laminar flowhood

A

Difference biosafety features

30
Q

High pressure inside the room than outside (Keeps the room clean - Clean room)

A

Positive pressure

31
Q

Low pressure in side than outside (Keeps the particulates inside - Dirty room)

A

Negative pressure

32
Q

Basic teaching research
GMT
Open work

A

Biosafety level 1

33
Q

Primary health services, diagnostic services, research

GMT, PPE, Biohazard sign

BSC for potential aerosols

A

Biosafety level 2

34
Q

Special diagnostic services

Airborne bacteria

Controlled access, Directional airflow

A

Biosafety level 3 (Containment)

35
Q

Dangerous pathogens (Ebola, Small pox, Etc,)

Class III BSC

A

Biosafety level 4

36
Q

Not A recognized containment level in biosafety guidance documents

Conducted in BSL 2 lab with Biosafety practices and procedures typically found at BSL 3

A

BSL 2+

37
Q

Assignment of microorganisms into groups based on: Pathogenecity, mode of transmission, preventive measures, treatment

A

Risk groups

38
Q

A microorganims that is unlikely to cause harm or disease

No or low individual and community risk

A

Risk group 1

39
Q

A pathogen than can cause human or animal disease but no serious to lab workers

Moderate individual risk, Low community risk

A

Risk Group 2

40
Q

Serious human or animal disease on one infected human (Blood borne pathogens)

high individual risks, Low community risk

A

Risk group 3

41
Q

Serious disease that can spread to the community, treatment may not be available

High on both individual and community risk

A

Risk group 4

Bacte Lab (Prelims) (7 decks)

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