3.1 Discussion

Question 1

Process of propagating organisms by providing the proper environmental nurtrients

Answer 1

Cultivation

Question 2

Refers to the separation of a strain from a natural, mixed population of living microbes

Answer 2

Isolation

Question 3

Term for bacteria that can be found everywhere

Answer 3

Ubiquity

Question 4

Term for bacteria that is where it grows and maintain viability in an artificial medium

Answer 4

Culture

Question 5

Cluster of cells

Answer 5

colony

Question 6

Single unadulterated specie of bacteria

Answer 6

Pure culture

Question 7

A solution containing these nutrients

Answer 7

Culture media

Question 8

Typed of culture media

Answer 8

Liquid
Solid
Semi solid

Question 9

Semi-solid and solid depends on?

Answer 9

amount of Solidifying agent

Question 10

Complex carbohydrate derived from seaweed

Answer 10

Agar

Question 11

Process of transferring microorganisms from one culture to another

Answer 11

Subculture

Question 12

Minimizes the likelihood that bacterial cultures will be contaminated

Answer 12

Aseptic technique

Question 13

2 or more bacteria in a single media called?

Answer 13

Mixed media

Question 14

What is the purpose of subculturing

Answer 14

1. To create a pure culture
2. To maintain viability

Question 15

Example of Flame sterilization equipment?

Answer 15

incinerator (Bacinerator)
Bunsen burner
Alcohol lamp

Question 16

Aseptically transfer microorganism from various media

Answer 16

Inoculating needles, loops

Question 17

What are the materials for inoculating loop?

Answer 17

Nichrome
Nickel-chromium
Platinum

Question 18

What is the standard size of inoculating loop?

Answer 18

3mm diameter

Question 19

What is the size require of inoculating loop in urine sample? why?

Answer 19

Smaller inoculating loop

To prevent too much bacteria in the loop when transferring to a media

Question 20

To dispense aliquots of inoculum, culture media or chemical reagents

Answer 20

Pipettes

Question 21

Transferring bacteria in a media

Answer 21

Inoculation

Question 22

medium containing bacteria

Answer 22

Inoculum

Question 23

2 types of serologic pipettes

Answer 23

To-Contain - blow out (2 rings)

To-Deliver - (No ring)

Question 24

What are the different automatic pipettes?

Answer 24

Automatic pipettes (Different sizes)

Repetitive pipettes

Multichannel pipette

Question 25

what are the 2 cultivation chambers?

Answer 25

Incubators
Water bath

Question 26

Temp for incubators?

Answer 26

37 (Dry heat)

Question 27

What is the term for freeze drying?

Answer 27

Lyophilization

Question 28

Temp for Ref?

Answer 28

4-10 C

Question 29

What is the purpose of storage in bacteria?

Answer 29

To minimize mutation caused by repeated subculturing

Question 30

How many months should store bacteria in a ref?

Answer 30

3 months

Question 31

Temp for freezers?

Answer 31

• 2-8C /20/80/110

Question 32

What reagent is used to preserve bacteria?

Answer 32

Glycerolw

Question 33

What environment is in freeze drying?

Answer 33

Lower temp (-80)

Pressure

Question 34

What is the results of lyophilization?

Answer 34

Turns into powder

Question 35

Techniques when streaking in different media

Answer 35

Broth - Gentle swirl
Slant - Streak
Butt / deep - Stab

Question 36

Techniques when streaking in agar plate

Answer 36

Streak plate
Spread plate
Pour plate

Question 37

What is the purpose of streak plate

Answer 37

Dilution technique

Question 38

What is the purpose of Spread and pour

Answer 38

Diluting to quantify bacteria in a sample

Question 39

What are the labels in the tube?

Answer 39

Date
Bacterial strains
Your initials