3.1 Discussion Flashcards
Process of propagating organisms by providing the proper environmental nurtrients
Cultivation
Refers to the separation of a strain from a natural, mixed population of living microbes
Isolation
Term for bacteria that can be found everywhere
Ubiquity
Term for bacteria that is where it grows and maintain viability in an artificial medium
Culture
Cluster of cells
colony
Single unadulterated specie of bacteria
Pure culture
A solution containing these nutrients
Culture media
Typed of culture media
Liquid
Solid
Semi solid
Semi-solid and solid depends on?
amount of Solidifying agent
Complex carbohydrate derived from seaweed
Agar
Process of transferring microorganisms from one culture to another
Subculture
Minimizes the likelihood that bacterial cultures will be contaminated
Aseptic technique
2 or more bacteria in a single media called?
Mixed media
What is the purpose of subculturing
- To create a pure culture
- To maintain viability
Example of Flame sterilization equipment?
incinerator (Bacinerator)
Bunsen burner
Alcohol lamp
Aseptically transfer microorganism from various media
Inoculating needles, loops
What are the materials for inoculating loop?
Nichrome
Nickel-chromium
Platinum
What is the standard size of inoculating loop?
3mm diameter
What is the size require of inoculating loop in urine sample? why?
Smaller inoculating loop
To prevent too much bacteria in the loop when transferring to a media
To dispense aliquots of inoculum, culture media or chemical reagents
Pipettes
Transferring bacteria in a media
Inoculation
medium containing bacteria
Inoculum
2 types of serologic pipettes
To-Contain - blow out (2 rings)
To-Deliver - (No ring)
What are the different automatic pipettes?
Automatic pipettes (Different sizes)
Repetitive pipettes
Multichannel pipette
what are the 2 cultivation chambers?
Incubators
Water bath
Temp for incubators?
37 (Dry heat)
What is the term for freeze drying?
Lyophilization
Temp for Ref?
4-10 C
What is the purpose of storage in bacteria?
To minimize mutation caused by repeated subculturing
How many months should store bacteria in a ref?
3 months
Temp for freezers?
- 2-8C /20/80/110
What reagent is used to preserve bacteria?
Glycerolw
What environment is in freeze drying?
Lower temp (-80)
Pressure
What is the results of lyophilization?
Turns into powder
Techniques when streaking in different media
Broth - Gentle swirl
Slant - Streak
Butt / deep - Stab
Techniques when streaking in agar plate
Streak plate
Spread plate
Pour plate
What is the purpose of streak plate
Dilution technique
What is the purpose of Spread and pour
Diluting to quantify bacteria in a sample
What are the labels in the tube?
Date
Bacterial strains
Your initials
