2.1 DISCUSSION Bacterial staining Flashcards
What microscope can visualize a living state of bacteria
Phase-contrast microscope
What is the use for simple stain?
- Morphological characterization (staph, strep)
What is Mixed culture?
2 or more types of bacteria in a culture or stain
What pairs with gram stain?
Culture and sensitivity
Why we cant stain chlamydia in gram stain?
It is located within the host cell
Why we cant stain Mycoplasma and ureaplasma in gram stain?
Lack cell wall
Why we cant stain Treponema in gram stain?
Insufficient dimension for light microscope
What is the purpose of mordant?
Kills and adheres to the slide and intensifies the color of the primary stain
What is under decolorization?
Alcohol is not left on long enough wo wash out the slide, resulting in Gram negative to appear gram positive
What is over decolorization?
Slide is left for to long in the alcohol resulting in gram positive may take in pink or gram neg
What is being dissolved in gram negative bacteria by alcohol?
Lipid layer
Coiling method requires training?
True
What is the purpose of heat in AFS (Ziehl neelsen)
penetrates stain to bacteria
What is the reagent for kinyoun that is replaced by ziehl neelsen?
Tergitol (penetration)
