3. Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms Flashcards
A culture containing a single organisms is called?
Pure culture
a medium that contains complex nutrients for the survival of microbes
culture medium
A medium that lacks solidifying agent
broth medium
Why use broth medium?
Useful for cultivating high numbers of bacterial cells in a small volume
A broth medium supplemented with solidifying agent is called?
agar
a complex carbohydrate
composed mainly of galactose, and is without nutritional value
Agar
What is the temp for agar when it liquifies?
100
What is the temp for agar when it solidifies?
40
What is the temp for growth of bacteria in an agar?
37.5
What is the concentration for solidified agar?
1.5-1.8%
What happens when the concentration of agar is less then 1%?
semisolid medium
medium that is useful for testing a cell’s ability
to grow within the agar at lower oxygen levels and for testing the species’ motility
Semisolid medium
a cluster of cells that originates
from the multiplication of a single cell and represents the growth of a single species of microorganism.
Colony
solid media in test tubes, which then cool and harden in a slanted position, producing
Agar slants
What medium is useful for maintaining pure culture?
Agar slants
What medium is primarily for studying gaseous requirements of microorganisms
Agar deep tubes/butt tubes
Liquid agar medium can also be poured into Petri dishes, producing _______, which provide
large surface areas for the isolation and study of microorganisms.
Agar plates
is the process
of rendering a medium or material free of all forms of life
Sterilization
hallmark of successful work in the microbiology laboratory
Sterility
This technique minimizes the likelihood that bacterial cultures will be contaminated, and reduces the
opportunity that you will be exposed to potential pathogens.
Aseptic technique
Petri dish only uses solid medium?
TRUE
What are the 2 material use to cultivate microorganism?
Test tubes and petri dishes
Who develop the first cotton plug?
Heinrich G. F Schroeder
Theodor von Dush
What is the modern version of cotton plug?
Morton Closures
Provides a large surface area for growth and cultivation
Petri dish
What contains the bottom part of the petri dish?
medium
After inoculation, petri dish is ______ to prevent condensation
Inverted
What do you call the transfer of bacteria?
sub-culturing
What instrument is used to transfer?
Wire loop and needles
What metal is used for wire loops and needles?
Nichrome and platinum
is useful for transferring a small volume of bacteria onto the surface of an agar
plate or slant
Wire loop
inoculate a culture into a broth medium or into an agar deep tube
Needle
What is the prime requirement for the growth of bacterias?
Temp
o maintain optimum temperature during the necessary growth
period
Incubator
Most incubators uses?
Dry heat
provides a rapid and uniform transfer of heat to the culture vessel,
and its agitation provides increased aeration, resulting in acceleration of growth
Shaking bath water
What is the disadvantage of shaking bath water?
Can only be use for broth medium
such as maintaining and storing stock cultures
between sub-culturing periods, and storing sterile media to prevent dehydration
Refrigerator
