3. Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms

Question 1

A culture containing a single organisms is called?

Answer 1

Pure culture

Question 2

a medium that contains complex nutrients for the survival of microbes

Answer 2

culture medium

Question 3

A medium that lacks solidifying agent

Answer 3

broth medium

Question 4

Why use broth medium?

Answer 4

Useful for cultivating high numbers of bacterial cells in a small volume

Question 5

A broth medium supplemented with solidifying agent is called?

Answer 5

agar

Question 6

a complex carbohydrate
composed mainly of galactose, and is without nutritional value

Answer 6

Agar

Question 7

What is the temp for agar when it liquifies?

Answer 7

100

Question 8

What is the temp for agar when it solidifies?

Answer 8

40

Question 9

What is the temp for growth of bacteria in an agar?

Answer 9

37.5

Question 10

What is the concentration for solidified agar?

Answer 10

1.5-1.8%

Question 11

What happens when the concentration of agar is less then 1%?

Answer 11

semisolid medium

Question 12

medium that is useful for testing a cell’s ability
to grow within the agar at lower oxygen levels and for testing the species’ motility

Answer 12

Semisolid medium

Question 13

a cluster of cells that originates
from the multiplication of a single cell and represents the growth of a single species of microorganism.

Answer 13

Colony

Question 14

solid media in test tubes, which then cool and harden in a slanted position, producing

Answer 14

Agar slants

Question 15

What medium is useful for maintaining pure culture?

Answer 15

Agar slants

Question 16

What medium is primarily for studying gaseous requirements of microorganisms

Answer 16

Agar deep tubes/butt tubes

Question 17

Liquid agar medium can also be poured into Petri dishes, producing _______, which provide
large surface areas for the isolation and study of microorganisms.

Answer 17

Agar plates

Question 18

is the process
of rendering a medium or material free of all forms of life

Answer 18

Sterilization

Question 19

hallmark of successful work in the microbiology laboratory

Answer 19

Sterility

Question 20

This technique minimizes the likelihood that bacterial cultures will be contaminated, and reduces the
opportunity that you will be exposed to potential pathogens.

Answer 20

Aseptic technique

Question 21

Petri dish only uses solid medium?

Answer 21

TRUE

Question 22

What are the 2 material use to cultivate microorganism?

Answer 22

Test tubes and petri dishes

Question 23

Who develop the first cotton plug?

Answer 23

Heinrich G. F Schroeder

Theodor von Dush

Question 24

What is the modern version of cotton plug?

Answer 24

Morton Closures

Question 25

Provides a large surface area for growth and cultivation

Answer 25

Petri dish

Question 26

What contains the bottom part of the petri dish?

Answer 26

medium

Question 27

After inoculation, petri dish is ______ to prevent condensation

Answer 27

Inverted

Question 28

What do you call the transfer of bacteria?

Answer 28

sub-culturing

Question 29

What instrument is used to transfer?

Answer 29

Wire loop and needles

Question 30

What metal is used for wire loops and needles?

Answer 30

Nichrome and platinum

Question 31

is useful for transferring a small volume of bacteria onto the surface of an agar
plate or slant

Answer 31

Wire loop

Question 32

inoculate a culture into a broth medium or into an agar deep tube

Answer 32

Needle

Question 33

What is the prime requirement for the growth of bacterias?

Answer 33

Temp

Question 34

o maintain optimum temperature during the necessary growth
period

Answer 34

Incubator

Question 35

Most incubators uses?

Answer 35

Dry heat

Question 36

provides a rapid and uniform transfer of heat to the culture vessel,
and its agitation provides increased aeration, resulting in acceleration of growth

Answer 36

Shaking bath water

Question 37

What is the disadvantage of shaking bath water?

Answer 37

Can only be use for broth medium

Question 38

such as maintaining and storing stock cultures
between sub-culturing periods, and storing sterile media to prevent dehydration

Answer 38

Refrigerator