4: Restriction Endonucleases: Detailed Table of RE's (MIDTERMS)

Question 1

Source, Recognition/cleavage site, Nature of cut ends of this RE:

EcoRI

Answer 1

Source: Escherichia coli RY13

Recognition/cleavage site:
‘5’ G AATTC 3’
3’ CTTAA G 5’

Nature of cut ends: Sticky 5’ overhang

Question 2

Source, Recognition/cleavage site, Nature of cut ends of this RE:

BamHI

Answer 2

Source: Bacillus amaloliquifaciens

Recognition/cleavage site:
5’ G GATTC 3’
3’ CCTAA G 5’

Nature of cut ends: Sticky 5’ overhang

Question 3

Source, Recognition/cleavage site, Nature of cut ends of this RE:

HindIII

Answer 3

Source: Haemophilus influenza Rd

Recognition/cleavage site:
5’ A AGCTT 3’
3’ TTCGA A 5

Nature of cut ends: Sticky 5’ overhang

Question 4

Source, Recognition/cleavage site, Nature of cut ends of this RE:

SalI

Answer 4

Source: Streptomyces albus G

Recognition/cleavage site:
5’ G TCGAC 3’
3’ CAGCT G 5’

Nature of cut ends: Sticky 5’ overhang

Question 5

Source, Recognition/cleavage site, Nature of cut ends of this RE:

SalI

Answer 5

Source: Streptomyces albus G

Recognition/cleavage site:
5’ G TCGAC 3’
3’ CAGCT G 5’

Nature of cut ends: Sticky 5’ overhang

Question 6

Source, Recognition/cleavage site, Nature of cut ends of this RE:

AluI

Answer 6

Source: Arthrobacter luteus

Recognition/cleavage site:
5’ AG CT 3’
3’ TC GA 5’

Nature of cut ends: Blunt end

Question 7

Source, Recognition/cleavage site, Nature of cut ends of this RE:

BalI

Answer 7

Source: Brevibacterium albidum

Recognition/cleavage site:
5’ TGG CCA 3’
3’ ACC GGT 5’

Nature of cut ends: Blunt end

Question 8

Source, Recognition/cleavage site, Nature of cut ends of this RE:

HaeIII

Answer 8

Source: Haemophilus aegyptius

Recognition/cleavage site:
5’ GG CC 3’
3’ CC GG 5’

Nature of cut ends: Blunt end

Question 9

Source, Recognition/cleavage site, Nature of cut ends of this RE:

SmaI

Answer 9

Source: Serratia marcescens

Recognition/cleavage site:
5’ CCC GGG 3’
3’ GGG CCC 5’

Nature of cut ends: Blunt end

Question 10

Source, Recognition/cleavage site, Nature of cut ends of this RE:

ApaI

Answer 10

Source: Acetobacter pasteurianus

Recognition/cleavage site:
5’ GGGCC C 3’
3’ C CCGGG 5’

Nature of cut ends: Sticky 3’ overhang

Question 11

Source, Recognition/cleavage site, Nature of cut ends of this RE:

ApII

Answer 11

Source: Arthrospira platensis NIES- 39

Recognition/cleavage site:
5’ CTGCA G 3’
3’ G ACGTC 5’

Nature of cut ends: Sticky 3’ overhang

Question 12

Source, Recognition/cleavage site, Nature of cut ends of this RE:

PstI

Answer 12

Source: Providencia stuartii

Recognition/cleavage site:
5’ CTGCA G 3’
3’ G ACGTC 5’

Nature of cut ends: Sticky 3’ overhang