1: Fundamentals of PCR (PCR table) Flashcards
1
Q
Temp
Initial denaturation
A
95
2
Q
Temp
Denaturation
A
94
3
Q
Temp
Annealing
A
55
4
Q
Temp
Extension
A
72
5
Q
Temp
Final extension
A
72
6
Q
Temp
Hold
A
4
7
Q
Time
Initial denaturation
A
5 min
8
Q
Time
Denaturation
A
1 min
9
Q
Time
Annealing
A
1 min
10
Q
Time
Extension
A
1 min
11
Q
Time
Final extension
A
1 min
12
Q
Time
Hold
A
Infinity
