4: PCR Variants (Isothermal) Flashcards
1
Q
Temperature
Nucleic acid sequence-based amplification (NASBA)
A
41 - 45
2
Q
Temperature
Strand displacement amplification
A
37
3
Q
Temperature
Rolling circle amplification
A
37
4
Q
Temperature
Recombinase polymerase amplification
A
37 - 42
5
Q
Temperature
Helicase-dependent amplification (HDA)
A
60 - 65
6
Q
Temperature
Polymerase spiral reaction
A
61 - 65
7
Q
Incubation time
Nucleic acid sequence-based amplification
A
1 - 2 hours
8
Q
Incubation time
Strand displacement amplification
A
15 mins
9
Q
Incubation time
Rolling circle amplification
A
1-2 hours
10
Q
Incubation time
Recombinase polymerase amplification
A
20 - 30 mins
11
Q
Incubation time
Helicase-dependent amplification
A
30 mins - 1 hour
12
Q
Incubation time
Polymerase spiral reaction `
A
30 - 60 mins
