3. Connective Tissue

Question 1

Where is Type 1 Collagen found?

Answer 1

Bone, Skin, Teeth, Tendons

Question 2

Where is Type II Collagen found?

Answer 2

Cartilage

Question 3

Where is Type III Collagen found?

Answer 3

(Reticulin) Bone marrow, Spleen, Liver

Question 4

Where is Type IV Collagen found?

Answer 4

Basement Membrane

Question 5

Where is Type V Collagen found?

Answer 5

Placenta

Question 6

What are glycosaminoglycans synthesised by?

Answer 6

Fibroblasts

Question 7

Is collagen elastic and contractile?

Answer 7

No

Question 8

Describe Individual collagen fibres.

Answer 8

Overlapping linear strands of tropocollagen (300nm) synthesised by fibroblasts, secreted by cells and assembled extracellularly.

Question 9

Describe tropocollagen

Answer 9

3 linear polypeptide chains wound in an a-helix. (2similar and 1 dissimilar chains)

Question 10

Extracellular fibres contain

Answer 10

Collagen
Reticulin
Elastin
In a jelly like matrix of glycosaminoglycans and proteoglycans

Question 11

Describe tendon

Answer 11

Thick parallel collagen fibres with squashed, blue fibroblasts between

Question 12

What is Reticulin?

Answer 12

Fibres which form Type 3 collage and stain black with silver stain

Question 13

Describe elastic tissue

Answer 13

Fibrillin microfibres in an amorphous matrix of elastin forms flat sheets
Stains pink with H&E with glassy appearance

Question 14

Which arteries contain elastin?

Answer 14

Closer to the heart is concentric sheets of perforated elastic tissue (seen clearly with Van Gieson - brown)

Question 15

2 types of adipose tissue

Answer 15

Connective tissue with fat
White = abundant large cells, each with a single fat droplet
Brown = Abundant in new born with multi-lobular cells containing numerous separate droplets of fat

Question 16

Cartilage structure

Answer 16

Rigid connective tissue derived from a common undifferentiated mesenchymal cell. Cartilage cells embedded in dense glycosaminoglycan matrix containing collagen and elastic tissue.
Chondroblasts trapped in matrix forms chondrocytes and secretes components of cartilage

Question 17

3 types of cartilage

Answer 17

Hyaline - GAG matrix contains collagen and elastic which stain glassy with H&E
Elastic. - Irregular fibres of elastic
Fibrous - Distinct bands of collagen

Question 18

Loose vs dense connective tissue

Answer 18

Loose = widely spaced thin collagen
Dense = closely spaced thick collagen