[2S] UNIT 8 Glucometer Flashcards by JULIA CASSANDRA RECCION

Point-of-care test for glucose conducted close to the site of patient care

Glucometer

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Working principle of a glucometer

Electrochemical

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Blood from a capillary puncture is introduced to a film strip comprised of several layers, each with unique function

Glucometer

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T/F: Enzymes or enzymatic reaction are better than colorimetric assays in detecting analytes

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Why is glucometer not the most ideal for glucose when it also detects enzymes?

We do not know how much enzymes are in a strip, that’s why we do not use this for FBS

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Enzymes such as glucose oxidase, hexokinase, and glucose dehydrogenase are used with reflectance or electrochemical detection

Glucometer

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The area of strip where it contains a reagent and also collects the blood sample. Majority where the chemical reaction takes place

Capillary Channel for Sample

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These monitoring devices are used in the home, in the physician’s office, or at the bedside in the hospital to monitor for hypoglycemia and hyperglycemia

In vitro diagnostic test systems: Glucometer

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Requirements for blood-glucose monitoring systems for self-testing in managing diabetes mellitus

In vitro diagnostic test systems: Glucometer

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T/F: Glucometer is for screening and confirming DM

F; NOT

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Another reason why glucometer is not for screening / confirmatory

Whole blood is used, specifically capillary blood. WB - the cells are still in contact w/ extracellular glucose, chances are they will try to metabolise glucose

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T/F: In a finger prick, the depth should not go beyond 2.4 mm, so a 2.2 mm depth maybe used

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Tends to give approx. 10%-15% lower glucose readings than plasma, but the percentage varies on the basis of hematocrit, analysis technique, and sample timing (fasting vs. postglucose load)

Whole Blood

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T/F: Most reference values rely on measurement of the serum / plasma

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T/F: In capillary puncture, always squeeze the punctured finger to release the blood

F, DO NOT SQUEEZE

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What finger should be pricked?

Ring / Middle finger

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ADVANTAGES OF A GLUCOMETER

T/F: Small blood volume

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T/F: Once the device touched the drop of blood, it will react with glucose oxidase, hexokinase, and glucose dehydrogenase

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ADVANTAGES OF A GLUCOMETER

T/F: Immediate results—no lab transportation

T/F: It is important to wipe the first drop of blood with cotton as it is contaminated with tissue fluid

ADVANTAGES OF A GLUCOMETER

T/F: Whole blood, urine, and other body fluids analyzed without processing

ADVANTAGES OF A GLUCOMETER

T/F: Narrow menu of tests available

F; Wide menu

ADVANTAGES OF A GLUCOMETER

T/F: Works within clinical patient flow

DISADVANTAGES OF A GLUCOMETER

T/F: More expensive than traditional laboratory tests

DISADVANTAGES OF A GLUCOMETER T/F: Quality is questionable as anyone can run the analysis

DISADVANTAGES OF A GLUCOMETER T/F: Easy regulatory compliance, billing, and documentation

F; Difficulties with regulatory compliance, billing, and documentation

DISADVANTAGES OF A GLUCOMETER T/F: Patient-focused staff with little formal education/experience in laboratory testing

Are routine tests that verify the reliability of a Glucometer (ACCU-CHEK® Performa meter) results at different ranges (using QC solution vials 1 and 2)

Quality Control Tests

Are routine tests that verify the test strip and QC solution integrity

Quality Control Tests

Are routine tests that verify the accuracy of the operator’s technique

Quality Control Tests

T/F: All operators must be able to perform QC tests. They can be done any time and are a good way to maintain your skill with the meter.

WHEN TO PERFORM A QC TEST T/F: Every 24 hrs

WHEN TO PERFORM A QC TEST T/F: Immediately before patient testing if the test has not been done within the last 24 hours.

WHEN TO PERFORM A QC TEST T/F: If the meter is not dropped

F; If the meter is dropped

WHEN TO PERFORM A QC TEST T/F: Every time you open a new container of test strips or a new vial of QC solutions

WHEN TO PERFORM A QC TEST T/F: If there is a question about a patient’s glucose results (i.e., if the glucose result is inconsistent with clinical presentation) to ensure the meter is working properly.

How many are the quality control solution vials?

2 vials (1 CN & 1 CP)

Supplies for QC Test

Glucometer Test strip container Quality Control solution vials (1 and 2)

T/F: Always check the expiration date on the test strip container and the QC vial to ensure that they have not expired.

APPLYING THE QC SOLN Mix the QC solutions by gently inverting the bottle _ times

APPLYING THE QC SOLN T/F: If you are opening the QC vial for the first time, write the date opened on the vial label.

APPLYING THE QC SOLN T/F: Always discard the 1st drop of QC solution

When enough QC solution is applied to the test strip, an ________ symbol flashes on the screen to show that the meter is analyzing the QC solution

hourglass

T/F: If results remain outside acceptable range after two attempts, do not use the meter for patient testing

Color of QC solution vial 1 cap

Grey

Color of QC solution vial 2 cap

White