[2S] UNIT 8 Glucometer Flashcards
Point-of-care test for glucose conducted close to the site of patient care
Glucometer
Working principle of a glucometer
Electrochemical
Blood from a capillary puncture is introduced to a film strip comprised of several layers, each with unique function
Glucometer
T/F: Enzymes or enzymatic reaction are better than colorimetric assays in detecting analytes
T
Why is glucometer not the most ideal for glucose when it also detects enzymes?
We do not know how much enzymes are in a strip, that’s why we do not use this for FBS
Enzymes such as glucose oxidase, hexokinase, and glucose dehydrogenase are used with reflectance or electrochemical detection
Glucometer
The area of strip where it contains a reagent and also collects the blood sample. Majority where the chemical reaction takes place
Capillary Channel for Sample
These monitoring devices are used in the home, in the physician’s office, or at the bedside in the hospital to monitor for hypoglycemia and hyperglycemia
In vitro diagnostic test systems: Glucometer
Requirements for blood-glucose monitoring systems for self-testing in managing diabetes mellitus
In vitro diagnostic test systems: Glucometer
T/F: Glucometer is for screening and confirming DM
F; NOT
Another reason why glucometer is not for screening / confirmatory
Whole blood is used, specifically capillary blood. WB - the cells are still in contact w/ extracellular glucose, chances are they will try to metabolise glucose
T/F: In a finger prick, the depth should not go beyond 2.4 mm, so a 2.2 mm depth maybe used
T
Tends to give approx. 10%-15% lower glucose readings than plasma, but the percentage varies on the basis of hematocrit, analysis technique, and sample timing (fasting vs. postglucose load)
Whole Blood
T/F: Most reference values rely on measurement of the serum / plasma
T
T/F: In capillary puncture, always squeeze the punctured finger to release the blood
F, DO NOT SQUEEZE
What finger should be pricked?
Ring / Middle finger
ADVANTAGES OF A GLUCOMETER
T/F: Small blood volume
T
T/F: Once the device touched the drop of blood, it will react with glucose oxidase, hexokinase, and glucose dehydrogenase
T