[2S] UNIT 6 Carbohydrates Flashcards

1
Q

Major food source and energy supply for the body

A

Carbohydrates

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2
Q

Where is carbohydrates stored as glycogen?

A

Liver & Muscle

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2
Q

Found in connective tissues and nucleic acids

A

Carbohydrates

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3
Q

FBS: increased in plasma glucose level seen in diabetic patient

A

Hyperglycemia

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4
Q

Produces ATP for active movement of substances

A

Carbohydrates

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5
Q

Increase level of glucose

A

Hyperglycemia

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6
Q

T/F: Hyperglycemia can be caused by factors like pancreatectomy, hemochromatosis and stress

A

T

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7
Q

T/F: Hyperglycemia can be caused by factors like mild infection, dehydration, pregnancy, and abnormal insulin receptor

A

F; severe infection

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8
Q

Decreased plasma glucose level

A

Hypoglycemia

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9
Q

Seen in diabetic patient who injects insulin

A

Hyperglycemia

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10
Q

T/F: Diabetic patient who injects insulin have 1 or 2 episodes of symptomatic hypoglycemia per week

A

T

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10
Q

Produced by B-cells of pancreatic islets of Langerhans

A

Insulin

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11
Q

Hormone that decreases plasma glucose levels

A

Insulin

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11
Q

Primary hormone responsible for the entry of glucose into the cell.

A

Insulin

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12
Q

Primary hypoglycemic agent

A

Insulin

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13
Q

Regulates glucose by increasing glycogenesis, lipogenesis, and glycolysis and inhibiting glycogenolysis

A

Insulin

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14
Q

Formation of glycogen from glucose

A

Glycogenesis

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15
Q

Process of fatty acid and triglyceride synthesis from glucose or other substrates

A

Lipogenesis

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16
Q

Breakdown of glucose in the formation of lactate, pyruvate, or other intermediate products such as ATP

A

Glycolysis

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16
Q

Breakdown of glycogen into glucose

A

Glycogenolysis

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17
Q

Primary hormone responsible for increasing glucose levels

A

Glucagon

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17
Q

Increases plasma glucose levels by glycogenolysis
and gluconeogenesis

A

Glucagon

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18
Q

Secreted by α-cells of pancreatic islets of Langerhans

A

Glucagon

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19
Q

Measured routinely using serum or plasma
○ Whole blood
○ Urine
○ CSF
○ Other body fluids

A

Glucose

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19
Formation of glucose from non-carbohydrate sources like amino acid, lactic, acid, glycerol, fats, and protein
Gluconeogenesis
20
T/F: In Glucagon, glucose is metabolized for the uptake of cells to other parts of the body
F; glycogen
21
Approximately 7 mg/dL lower than capillary blood due to tissue metabolism
Venous blood glucose
21
Approximately 11% lower than serum or plasma glucose
Whole blood glucose
22
T/F: Glycolysis lowers the glucose levels in an uncentrifuged blood specimen
T
22
Approximately 60-70% plasma glucose levels
CSF glucose levels
23
Obtained in the morning (8-10 hrs fasting, not longer than 16 hrs)
Fasting Blood Glucose
24
Can inhibit in vitro glycolysis
Sodium Fluoride Tube (Gray)
24
T/F: Fasting Plasma Glucose has diurnal variation: ↑ AM than in PM
T
25
METHODS OF GLUCOSE DETERMINATION Alkaline copper reduction method
Non-Enzymatic
25
Sodium Fluoride Tube (Gray) is stable for up to _ days at room temp
3
26
METHODS OF GLUCOSE DETERMINATION Condensation method
Non-Enzymatic
26
METHODS OF GLUCOSE DETERMINATION Alkaline ferric reduction method
Non-Enzymatic
27
METHODS OF GLUCOSE DETERMINATION Glucose oxidase
Enzymatic
28
METHODS OF GLUCOSE DETERMINATION Hexokinase
Enzymatic
29
Principle: Reduction of cupric ions to cuprous ions forming cuprous oxide in hot alkaline solution by glucose ○ Redox reaction
Alkaline Copper Reduction Method
29
Glucose + alk. cupric tartrate → CuOH + heat → cuprous oxide
ACR: Nelson Somogyi Method & Folin Wu
29
METHODS OF GLUCOSE DETERMINATION Glucose dehydrogenase
Enzymatic
30
Most commonly used chemical method
ACR: Nelson Somogyi Method
31
Cu2O + arsenomolybdic acid → arsenomolybdenum blue
ACR: Nelson Somogyi Method
32
○ Add Barium sulfate (BaSO4) when preparing Protein-Free Filtrates to remove saccharoids (non-glucose reducing substances) ○ Measures true blood glucose
ACR: Nelson Somogyi Method
33
Glucose + alk. cupric tartrate → CuOH + heat → cuprous oxide
ACR: Folin Wu Method & Nelson Somogyi Method
34
Cu2O + phosphomolybdic acid → phosphomolybdenum blue
ACR: Folin Wu Method
35
Glucose + alk. cupric-neocuproine → Cuprous-neocuproine complex
ACR: Neocuproine Method
36
Glucose + CuSO4 (blue) + heat → Cu2O
ACR: Benedict's Method
36
2,9-dimethyl-1,10-phenanthroline-8-cl
ACR: Neocuproine Reagent Formula
36
End color: yellow to yellow-orange
ACR: Neocuproine Method
37
Uses tartrate & citrate as stabilizing agent
ACR: Benedict's Method
38
End color: green → yellow → orange → brick red
ACR: Benedict's Method
39
Principle: Reduction of ferricyanide to ferrocyanide in hot alkaline solution by glucose
Alkaline Ferric Reduction Method
39
Glucose + [Fe(CN)6] 3 − (yellow) in alk soln + heat → [Fe(CN)6]4− (colorless)
AFR: Hagedorn Jensen Method
40
End color: bluish green
Ortho-Toluidine Method
40
Inverse colorimetry
AFR: Hagedorn Jensen Method
41
Glucose + o-toluidine + acetic acid + heat → N-glucosylamine
Ortho-Toluidine Method
41
Principle: Ability of primary aromatic amine acid solution to condense with the aldehyde group of glucose to form glycosylamines (Schiff bases)
Condensation Method
41
Main interfering substances in condensation method
○ Galactose (aldohexose) ○ Manose (aldopentose)
42
● Also called Saifer Gernstenfield method ● One of the most used in blood glucose determination ● Prone to errors
Glucose Oxidase Method
43
● Step 1: Mutarotase catalyzes mutarotation of α- to β-glucose ● Step 2 & 3: Trinder reaction – couple reaction
Glucose Oxidase Method
44
Considered as the reference method for blood glucose determination
Hexokinase Method
45
tetrazolium bromide salt (colorless)
MTT (glucose dehydrogenase)
45
Increased or Decreased Carbo? Diabetes Mellitus
Increased
46
Increased or Decreased Carbo? Pancreatitis
Increased
46
Increased or Decreased Carbo? Pituitary or thyroid dysfunction
Increased
47
Increased or Decreased Carbo? Renal failure
Increased
48
Increased or Decreased Carbo? Insulin-induced hypoglycemia
Decreased
48
Increased or Decreased Carbo? Liver disease
Increased
49
Increased or Decreased Carbo? Starvation
Decreased
49
Increased or Decreased Carbo? Hyperinsulinemia
Decreased
50
Increased or Decreased Carbo? Neoplasms
Decreased
51
Conversion factor of mg/dL to mmol/L
0.0556
51
Conversion factor of mmol/L to mg/dL
18
52
Tubes used in Glucose Oxidase Method
Kahn Tubes
53
Enzyme reagents of the glucose reagent kit
4 PP ni GPS 4-aminoantipyrine Phosphate buffer (pH 7.4) Phenol Glucose oxidase Peroxidase Sodium azide
54
What to pipet first? a. Blank b. Standard c. Normal Control d. Pathologic Control e. Unknown
b. Standard
55
GLUCOSE OXIDASE METHOD Where to pipet when purging the standard?
Deliver it into the tube by touching the tip of the pipet directly to the bottom of the tube
56
GLUCOSE OXIDASE METHOD How to pipet the reagent in the tube?
By touching the tip of the pipet to the side of the tube
57
GLUCOSE OXIDASE METHOD PROCEDURE 7: Mix the solution by covering the mouth of the test tube with a ________ and inverting the tubes _____.
parafilm 2-3x
58
GLUCOSE OXIDASE METHOD PROCEDURE 8: Incubate for ______ at _____ water bath.
5 minutes at 37°C
59
GLUCOSE OXIDASE METHOD: ASSAY REQUIREMENTS Wavelength
500 nm
60
GLUCOSE OXIDASE METHOD: ASSAY REQUIREMENTS Optical Path
1 cm
61
GLUCOSE OXIDASE METHOD: ASSAY REQUIREMENTS Temperature
37°C
62
GLUCOSE OXIDASE METHOD: ASSAY REQUIREMENTS Measurement
Read against reagent blank
63
GLUCOSE OXIDASE METHOD PROCEDURE 10: Measure absorbance of all tubes within ___ minutes
60 minutes
64
Reference Value of Glucose
CU = 70-110 mg/dL SI = 3.9 - 6.1 mmol/L