2S [LAB]: Reticulocyte Flashcards
The last immature RBC stage
Reticulocyte
Normally, a reticulocyte spends how many days in the bone marrow?
1-2
A reticulocyte spends how many days in the peripheral blood before developing into a mature RBC
1
Anticoagulant of choice in reticulocyte count
EDTA
The small network of basophilic materials in the reticulocytes
Reticulum
Reticulocyte count is used as an index of ___ and ___
Bone marrow activity and RBC production
Method of reticulocyte count:
Rapid method of Schilling
Wet method
Method of reticulocyte count:
Osgood-Wilhelm and Sabin
Wet method
Method of reticulocyte count:
New methylene blue
Dry method
Method of reticulocyte count:
Cook
Dry method
Method of reticulocyte count:
Meyer
Dry method
Method of reticulocyte count:
Tureen
Dry method
Method of reticulocyte count:
Seiverd
Dry method
Ratio of blood to filtered stain in reticulocyte
1:1
Incubation time in reticulocyte count after adding the stain
3-10 mins (ppt: 15-30 mins)
Magnification used in reticulocyte count
OIO (1000x)
T/F: Babies have a higher reticulocyte count than adults
True
Reference range of reticulocyte count in adults
0.5-1.5%
Reference range of reticulocyte count in children
2.0-6.0%
Patients with a hematocrit of 35% should have an elevated corrected reticulocyte count of ___
2-3%
Patients with a hematocrit of 25% should have an elevated corrected reticulocyte count of ___
3-5%
Reference range of absolute reticulocyte count
20x10^9 to 115x10^9/ L
An RPI of ___ indicates adequate BM response
3
An RPI of ___ indicates inadequate BM response
2
Hct of patient if the maturation time of reticulocyte is 1 day
40-45%
Hct of patient if the maturation time of reticulocyte is 1.5 days
35-39%
Hct of patient if the maturation time of reticulocyte is 2 days
25-34%
Hct of patient if the maturation time of reticulocyte is 2.5 days
15-24%
Hct of patient if the maturation time of reticulocyte is 3 days
<15%
Remedy in reticulocyte count if the blood is anemic and polycythemic
Adjust blood and dye proportion
Moisture and poor drying in reticulocyte count causes the formation of ___
Refractile bodies
RBC inclusions confused with reticulocyte
Heinz bodies
Pappenheimer
Howell Jolly
Using the Miller Disc in reticulocyte requires the use of ___ rule
Edge rule
Precipitated hemoglobin that usually appear round or oval and tend to adhere to the cell membrane that is confused as reticulocyte
Heinz bodies
Singular round nuclear fragments that is confused as reticulocyte
Howell-Jolly bodies
Iron in the mitochondria whose presence can be confirmed with an iron stain that is confused as reticulocyte
Pappenheimer bodies
When reticulocytes are released earlier to compensate for anemia
Shift reticulocyte
In RPI determination, correction should be made if ___ is reported in the RBC morphology
Polychromasia
Shifted reticulocytes stay how much longer in the peripheral blood circulation?
more than 1 day