2.9 Photosynthesis Flashcards

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1
Q

Photosynthesis

A

Process through which solar light energy is captured by chlorophyll and converted into chemical bond energy stored in organic molecules using water and carbon dioxide

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2
Q

Which is the shortest and longest wavelength in visible light spectrum?

A
Shortest= violet
Longest = red
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3
Q

What are the wavelengths our eyes can detect?

A

Between 400-700nm

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4
Q

Chloroplast

A

An organelle that contains chlorophyll (green pigment) and traps light energy

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5
Q

Where is chloroplast located?

A

Mesophyll cells and guard cells

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6
Q

Which is the primary pigment?

A

Chlorophyll A

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7
Q

Which light does chlorophyll A absorbs most and least effectively?

A

Absorbs red and blue most effectively. Doesn’t absorb green light at all

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8
Q

What light does chlorophyll A reflect?

A

Green

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9
Q

Difference between absorption and action spectrum

A

Absorption: Shows which wavelength is absorbed by a particular photosynthetic pigment
Action: Shows efficiency of rate of photosynthesis at each wavelength in visible spectrum

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10
Q

Which peak is higher in absorption spectrum: blue or red?

A

Blue as it’s absorbed more

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11
Q

Accessory pigments

A

Allows some plants to take advantage of green wavelength. Beneficial for underwater plants

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12
Q

Why do leaves turn brown in the fall?

A

Active chlorophyll has accessory pigments that cannot be seen but when temperatures cool, those pigments are broken down and hence shown

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13
Q

Photolysis

A

Stage of photosynthesis where water is split by light

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14
Q

What does 2H2O+energy in photons give?

A

4e-, 4H+ and O2

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15
Q

Where do the electrons, H ions and O2 go after photolysis?

A

Electrons and hydrogen ions are used later in photosynthesis, oxygen is a waste product that diffuses out

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16
Q

Is photosynthesis anabolic or catabolic?

A

It is anabolic (small to large molecules)

17
Q

What is light-dependent reaction? Where does it occur?

A

Requires the use of light to split water and create ATP, making H+ ions. Occurs in the thylakoids

18
Q

What is light independent reactions and where do they occur?

A

Occurs without light and uses ATP and H+ ions to ‘fix’ carbon dioxide making glucose. Takes place in the stroma of chloroplasts

19
Q

What is carbon fixation also known as?

A

Calvin cycle

20
Q

Steps of light-independent reaction

A
  1. H ions + NADP= NADPH
  2. e- + ADP = ATP
  3. ATP and NADPH move to Calvin cycle
  4. CO2 is used to form complex sugars
  5. Calvin cycle gives ADP and NADP back to light reactions
21
Q

Limiting factor

A

Factor that restricts rate of reaction when present in low amounts

22
Q

Why will photosynthesis plateau at a certain light intensity?

A

Photosynthesis operates with a certain number of photosystems that capture photons. If they are all occupied, rate cannot increase

23
Q

Why will photosynthesis plateau at a certain carbon dioxide conc.?

A

Once all active sites of the enzymes are occupied with a substrate, any increase in CO2 will not increase the rate

24
Q

Relationship between temperature and photosynthesis and reason

A

Rate will increase as temperature increases however past optimum temperature, enzymes start to denature and rate decreases. Graph will show a peak

25
Q

Chromatography

A

Technique used to separate mixture of substances based on movement of different constituents on piece of paper by capillary action

26
Q

What phase is paper and solvent?

A

Paper is stationary phase while solvent is mobile phase

27
Q

Retention factor

A

Ratio of distance moved by pigment to distance moved by solvent

28
Q

What can Rf be used to identify in photosynthesis

A

The different chlorophyll pigments

29
Q

Thin layer chromatography + advantages

A

Uses silica gel or cellulose instead of paper. Gives better result as it’s well defined.