2.3 Biological Molecules Flashcards
What are lipids?
a group of biological molecules including fats and oils, made from fatty acid chains and glycerol
What elements do lipids contain?
Carbon
Hydrogen
Oxygen
How do you test for fat (lipids)?
- add 2cm3 of ETHANOL to the test solution
- add 2cm3 of distilled water
- leave for 3 minutes and then record the colour
–> if fat is present a milky white emulsion will form
–> if fat is not present the solution will remain colourless
Which chemical elements are present in carbohydrates?
- carbon
- oxygen
- hydrogen
CHO
Which chemical elements are present in proteins?
- carbon
- oxygen
- hydrogen
- sulphur
- nitrogen
- phosphorus
CHONPS
Which molecules are carbohydrates broken down into?
The polymers break down into simple sugars
Which molecules are proteins broken down into?
amino acids
Which molecules are lipids broken down into?
The large polymers break down into 3 fatty acids and glycerol
How to test for glucose?
- Add BENEDICT’S SOLUTION into the test tube containing the food sample
- Heat in a water bath at 60-70 degrees for 5 minutes
- Take test tube out and record the colour
–> if glucose is present the solution will turn brick red
–> if glucose is not present solution will remain blue
How to test for starch?
- Pipette the sample solution into wells or on a tile
- add drops of IODINE SOLUTION and leave for 1 minute
- record any colour change
–> if starch is present the solution will turn blue-black
–> is starch is not present the solution will remain brown
How to test for protein?
- add the sample solution into a test tube
- add drops of BIURET SOLUTION into the test tube
- leave for 1 minute and then record the colour
–> is protein is present the solution will turn purple
–> if protein is not present the solution will remain blue
What are amino acids?
the monomer from which proteins are assembled from
Glycogen
a carbohydrate made from glucose that acts as an energy store in animals
enzymes
proteins that are biological catalysts that increase the rate of reactions by providing an alternate pathway with a lower activation energy
Active site
part of the enzyme that is complementary to the shape of the substance which can change shape if the enzyme is exposed to extreme pH solutions or high temperatures
–> this would result in less enzyme-substrate complexes