2.2.2 Staining Methods (SEM) Flashcards
Uses aqueous or alcoholic solutions of dye
Direct staining
Staining technique that employs use of mordant and accentuator
Indirect staining
Indirect staining:
A substance that serves as link between the dye and the tissue? Ex?
Mordant:
Potassium alum
Iron
Indirect staining:
Substance that increases staining power of the dye
It heightens color intensity of the dye
ex?
Accentuator:
KOH
Phenol
Tx + dye = color
Direct staining
Tx + dye + M or A = color
Indirect staining
Mayers hematoxylin, Ehrlich;s hematoxylin, Harris Hematoxylin
These are an example of?
Forms of Hematoxylin with K-ALUM as MORDANT
ALUM hematoxylin
Weigert’s hematoxylin, Heidenhain’s hematoxylin
Forms of hematoxylin with IRON as MORDANT
IRON hematoxylin
Involves gradual application of dye
Follows definite sequence
No excess dye
No decolorization needed
Progressive staining
Regressive staining involves
Overstaining the tissue
There is Excess dye
We do decolorization
T or F
T
Selective removal of excess dye
Decolorization / Differentiation
Decolorization / Differentiation most commonly used?
Acid alcohol
Staining technique that involves use of dye/stain that gives a different color, different from the color of the dye itself
Metachromatic staining
Mainly used for demonstration of cartilage CT, epithelial mucins, mast cells granule, amyloid
Metachromatic staining
Methyl Violet
Crystal violet
Safranin
Bismarck Brown
Basic fuchsin
Methylene blue
Thionine
Toluidine Blue
Azure A, B, C
Cresylblue
These are all examples of?
Metachromatic staining
Staining technique in which tissue components are stained with the same shade or hue as that of the dye?
Orthochromasia
Staining method that involves the use of another dye to provide contrast and background
Counter staining
Counter staining:
Most Commonly used?
Eosin
Tissue components are demonstrated not by stains but by colorless solutions of metallic salts that produces black deposits on the surface of tissues
Metallic impregnation
Examples of agents used in metallic impregnation?
Gold chloride
Silver nitrate
Extra:
Ammonia cal Silver
Staining of living cell components involves cytoplasmic phagocytosis
Vital staining
Methods of Vital staining
Intravital
Supravital
Injecting the dye to any part of the living body
Intravital staining
Involves immediate application of stain to living cells following removal from living body
Supravital staining
The best vital dye
Neutral red (Supra)
Supravital stain recommended for mitochrondia
Janus green
Neutral red, Janus green, trypan blue, Nile blue, thionine, toluidine blue
These dyes belong to what stains?
Supravital stain
