1.10.1 Dehydration Flashcards
Routine Dehydrating agent
Ethyl alcohol
Important requirement when using alcohol as dehydrating agent
Use of ascending/Increasing conc. of alcohol
NOTE:
The ascending conc of Alcohol:
70% , 80% , 95%
This prevent destruction of tissue, para di mabigla
For Dehydrating sections and smears
Methyl alcohol (Methanol
Fast acting, non toxic
Ethyl alcohol
Methanol - Toxic
Toxic by ingestion, inhalation and by skin contact
Highly corrosive on skin
Toxic to reproductive, fetal, urinary system
Cellosolve (Ethylene glycol monoethyl ether)
Dehydrating agents:
1. Alcohol
2. Cellosolve
3. Dioxane
4. THF
T or F
T
Dehydrating agent whose main disadvantage is its being toxic
Dioxane (Diethylene dioxide)
Decomposes whe expose to sunlight
Cellosolve
Temp that will hasten dehydration time
37 C
Dehydrating agent that is both a skin and eye irritant
Has an offensive odor
Tetrahydrofuran THF
Rapid-acting dehydrating suited for urgent biopsies but not for routine tissue processing
Acetone
Acetone dissolves easily and dissolves fats
T or F
T
Alcohol for plant and animal microtechniques
Butyl alcohol
Purpose of phenol in alcohol bath?
Soften hard Tissues