1.12.1 Infiltration and Embedding Flashcards
Remove clearing agent and to fill up cavities of tissue space
Impregnation / Infiltration
Most common reagent for Infiltration?
Paraffin wax
Manual = Oven
Automatic = Autotechnicon
Vacuum
T or F
T
2 methods for cellodin infiltration:
Wet
Dry
Gelatin infiltration method = For studies
T or f
T
Paraffin wax infiltration:
Melting point commonly used?
56 C
Paraffin wax infiltration:
Not suited for?
Fatty tissues
Paraffin wax infiltration:
Manual method requires __________ changes of Wax
4
Paraffin wax infiltration:
Automatic method requires __________ changes of wax
2-3
Substitute for paraffin wax recommendation for bones and brain specimen
Paraplast
Paraffin wax is a Rapid reagent due to heat
T or F
T
Substitute for paraffin wax that is water soluble and is recommended for enzyme histochemical studies
Carbowax
Temperature range of paraffin oven when in used
55-60C
Approximate temperature of paraffin Oven when in used
2-5 deg C higher than the wax melting point
Infiltration under negative atmospheric pressure inside the oven
Vacuum infiltration
Substitute for paraffin wax used for eye specimens
Bioloid
Substitute for paraffin wax that will require heavy duty type of microtome, harder than P. wax
Esterwax
Melting point of paraplast
56 - 57
Melting point of Ester wax
46-48
Celloidin infiltration:
Disadvantage?
Slow due to no heat
Celloidin infiltration:
Methods:
____________ for eyes. Uses GILSON’s mixture to store blocks
Dry
Celloidin infiltration:
Methods:
____________ for Bones, brain, and whole organ
Wet
Celloidin infiltration:
Wet Method uses ______________ tp store the block prior to sectioning
70-80% alcohol
Infiltration method for enzyme and histochemical studies
Carbowax
Celloidin infiltration:
Methods:
Composition of Gilson’s mixture? use to store prior to sectioning
Chloroform and Cedarwood oil
Infiltration method that will require tissues not to be more than 2-3 mm thick
Gelatin infiltration
A disposable embedding mold that will require smearing of the inner mold with glycerine
Plastic ice trays
An embedding mold consisting of a series of interlocking plates resting on a flat metal base
Compound embedding unit
Disposable mold that gives perfect blocks without trimming
Peel-away mold
COmpound embedding unit advantage:
Can embed more than 1 tissues
T or F
T
Other term for embedding:
Blocking
Casting
T or F
T
Process of infiltrating tissu with celloidin and embedding with paraffin
Double embedding
Embedding mold consisting of L shaped strips made of heavy brass
Leukchart’s
Use of Phenol in gelatin infiltration
To prevent growth of mold
Celloidin infiltration:
Thin: ____
Medium :____
Thick:_____
2-4%
4-6%
8-12%
Plastic resins include:
Spurs
Polyester
Polyglycl methacrylate / GMA
Methylmethcrylate / MMA
T or F
T
Fastest Epoxy resin?
Spurr
Plastic resin originally introduced for EM
Polyester
Extremely hydrophilic
Polyglycl methacrylate / GMA
Methylmethcrylate / MMA
Polyglycl methacrylate / GMA
Plastic resin considered ideal for undecalcified bones and hard tissues
Polyglycl methacrylate / GMA
Methylmethcrylate / MMA
Methylmethcrylate / MMA
what is the product of embedding?
Tissue block
