1.5 Exfoliative Cytology Flashcards
Most common specimen in histopath for Exfoliative cytology
Pap’s Smear
Involves the study of cells SHED from epithelial surfaces
Cytology
Who collect Pap’s Smear
OB gynecologist
Who process Pap’s smear?
Medtech
Who analyzes Pap’s smear?
Pathologist
What is the routine fixative for cytology
95% ethanol
What is the routine tissue fixative??
10% buffered formaline
What is the usual stain for cytology?
Papniculao stain
What is the tissue stain?
Hemotoxylin and Eosin stain (H and E)
3 stains of papaniculao stain?
Harris Hematoxylin
OG-6
EA 50
Primary dye in Pap’s, nuclear stain
Harris Hematoxylin
Counter stain in Pap’s Used to stain Cytoplasm of all superficial cells
OG-6
Counter stain in Pap’s Used to stain Cytoplasm of intermediate and Parabasal cell
EA 50
PAP’s smear microscopy (Vaginal cells):
Largest cell to smallest size?
Superficial
Intermediate
Parabasal cells
Basal cells
PAP’s smear microscopy (Vaginal cells):
Largest cell and smallest NUCLEUS?
Superficial