1.8.3 Fixative Con. Flashcards
For brain tissues for the diagnosis of rabies
Acetone, carnoy’s
Excellent for Glycogen Demonstration
Picric Acid Fixatives
For Embryos, pituitary biopsies and Endometrial curetting’s NOT for kidney structures
NOT COMPATIBLE with Feulgen’s
Bouin’s fluid
For Touch prep
95% Isopropyl alcohol
Solidifies at 17 C
Glacial HAC
Must be used at ice cold temp
FOR enzymes
FIXATIVE and DEHYDRATING agent
Acetone
For mucopolysaccharides and nuclear protein
Newcomer’s (Alcohol fixatives)
Most rapid tissue fixative for fixing chromosomes and Lymph glands
Carnoy’s (Alc. fixative)
Osmium Tetroxide, Glutaraldehyde, and Paraformaldehyde are use for what microscope?
Electron micrscope
Fixation method that involves thrmal coagulation of proteins
Heat fixation
Fixation folume for museum preparation should not be LESS THAN ____________
50-100x vol of Specimen
Fixatives for enzyme histochemistry:
4% formaldehyde
Formol saline
Which of the following?
All
Smallest aldehyde
Glyoxal
Glyoxal is a rapid aldehyde fixative
t or f
T
Main ingredient for Bouin’s Brasil and Hollande’s
Picric acid fixative
Can be used for myelin and pripheral nerves
Osmium tetroxide
Osmium tetroxide is not usually for routine fixative due to ________ acting fixative, expensive and req 5-10x vol of the specimen
Slow
Proposed as mercuric chloride substitute
ZnSO4 (Zinc sulfate)
Can be used as fixative, decalcifying agent and stain
Picric acid
Often used in conjunction with other fixatives to produce compound fixative
Glacial Acetic acid (HAc)
Alcohol and aldehyde is use for dehydration and fixation
T or F
T
Picric acid stain imparts what color?
Yellow
