2.2 Haemoglobin Spectrophotometry And Electrophoresis

Question 1

What is haemoglobin made up of

Answer 1

Protein components globin chains and a haem group covalently linked

Question 2

What is the haem group

Answer 2

Porphyria ring bound to ferrous ion (Fe2+)

Question 3

Hb A

Answer 3

Quaternary, mostly helical

Hydrophilic surface and phonic core

Haem groups in pocket of globin chains (4 per molecule of HbA) - each haem group can bind one O2 molecule

Has 2alpha and 2beta globin units

Question 4

Hb F

Answer 4

Foetal

2alpha 2gamma subunits

Greater affinity for O2 and reduced affinity for 2,3BPG

Question 5

What modifies the binding of O2

Answer 5

Binding of the metabolite 2,3-BPG to Hb

Found in RBC in same conc as Hb

Reduces affinity for O2

Allosteric effector

Question 6

Lungs pp compared to tissue

Answer 6

Lungs 100 torr readily saturates w O2

Tissues pp much lower, Hb releases most O2

Question 7

Beer lambert law

Answer 7

A = E x c x l

A= absorbance
C= conc of absorbing substances
L= path length
E= extinction coefficient

Question 8

Why is the first drop of blood wiped away

Answer 8

Has cytosol and extra cellular fluid where cells are broken by lancate

Question 9

Mcv

Answer 9

Mean cell volume

Question 10

Mcv calculation

Answer 10

Hct/ RBC =…L

Question 11

Mch

Answer 11

Mean cell Hb

Average mass of Hb in each RBC

Question 12

Mch calculation

Answer 12

Hb/ rbc =…g

Question 13

Mchc

Answer 13

Mean cell Hb conc

Question 14

Mchc calculation

Answer 14

Hb/ hct=…g/L